PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G35460.1
Common NameBHLH80, EN71, F12A4.2, FBH1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 259aa    MW: 28526.6 Da    PI: 5.2259
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G35460.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH40.54.8e-13193238655
                  HHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   6 nerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  +++Er RR ri +++ +L+el+P+     +k  + a++Le+AveY+k Lq
  AT1G35460.1 193 SIAERVRRTRISDRIRRLQELVPNM----DKQTNTADMLEEAVEYVKALQ 238
                  689*********************7....688*****************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088814.837187237IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474591.31E-16190253IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000834.42E-12191242No hitNo description
Gene3DG3DSA:4.10.280.105.5E-16192253IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003536.9E-13193243IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000103.8E-10193238IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0042335Biological Processcuticle development
GO:0045944Biological Processpositive regulation of transcription from RNA polymerase II promoter
GO:0005634Cellular Componentnucleus
GO:0001046Molecular Functioncore promoter sequence-specific DNA binding
GO:0001228Molecular Functiontranscriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0005515Molecular Functionprotein binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000293anatomyguard cell
Sequence ? help Back to Top
Protein Sequence    Length: 259 aa     Download sequence    Send to blast
MQSTHISGGS SGGGGGGGGE VSRSGLSRIR SAPATWIETL LEEDEEEGLK PNLCLTELLT  60
GNNNSGGVIT SRDDSFEFLS SVEQGLYNHH QGGGFHRQNS SPADFLSGSG SGTDGYFSNF  120
GIPANYDYLS TNVDISPTKR SRDMETQFSS QLKEEQMSGG ISGMMDMNMD KIFEDSVPCR  180
VRAKRGCATH PRSIAERVRR TRISDRIRRL QELVPNMDKQ TNTADMLEEA VEYVKALQSQ  240
IQELTEQQKR CKCKPKEEQ
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.180170.0flower
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO306931940.0
Genevisible260079_s_at2e-34
Genevisible252798_at2e-34
Expression AtlasAT1G35460-
AtGenExpressAT1G35460-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems, and flowers. {ECO:0000269|PubMed:12679534}.
Function -- GeneRIF ? help Back to Top
  1. There are four CONSTANS transcriptional activators FBH1-4 which are related basic helix-loop-helix-type transcription factors that preferentially bind to the E-box cis-elements in the CO promoter. Overexpression of all FBH genes drastically elevated CO levels and caused early flowering regardless of photoperiod. [FBH1]
    [PMID: 22334645]
  2. Results indicate a role for FLOWERING BASIC HELIX-LOOP-HELIX 1 (FBH1, AT1G35460) as a transcriptional modulator of warm temperature signals and clock responses.
    [PMID: 25246594]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00181DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G35460.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G35460
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4117910.0AF411791.1 Arabidopsis thaliana At1g35460/F12A4_2 mRNA, complete cds.
GenBankAY0937940.0AY093794.1 Arabidopsis thaliana At1g35460/F12A4_2 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_174776.10.0basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ9C8P80.0BH080_ARATH; Transcription factor bHLH80
TrEMBLA0A178WC480.0A0A178WC48_ARATH; FBH1
STRINGAT1G35460.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM51042551
Representative plantOGRP5111580
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  3. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Nawy T, et al.
    Transcriptional profile of the Arabidopsis root quiescent center.
    Plant Cell, 2005. 17(7): p. 1908-25
    [PMID:15937229]
  7. Kuusk S,Sohlberg JJ,Magnus Eklund D,Sundberg E
    Functionally redundant SHI family genes regulate Arabidopsis gynoecium development in a dose-dependent manner.
    Plant J., 2006. 47(1): p. 99-111
    [PMID:16740146]
  8. Panjabi P, et al.
    Comparative mapping of Brassica juncea and Arabidopsis thaliana using Intron Polymorphism (IP) markers: homoeologous relationships, diversification and evolution of the A, B and C Brassica genomes.
    BMC Genomics, 2008. 9: p. 113
    [PMID:18315867]
  9. Elrouby N,Coupland G
    Proteome-wide screens for small ubiquitin-like modifier (SUMO) substrates identify Arabidopsis proteins implicated in diverse biological processes.
    Proc. Natl. Acad. Sci. U.S.A., 2010. 107(40): p. 17415-20
    [PMID:20855607]
  10. Ito S, et al.
    FLOWERING BHLH transcriptional activators control expression of the photoperiodic flowering regulator CONSTANS in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2012. 109(9): p. 3582-7
    [PMID:22334645]
  11. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  12. Nagel DH,Pruneda-Paz JL,Kay SA
    FBH1 affects warm temperature responses in the Arabidopsis circadian clock.
    Proc. Natl. Acad. Sci. U.S.A., 2014. 111(40): p. 14595-600
    [PMID:25246594]
  13. Li S, et al.
    CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis.
    PLoS Genet., 2016. 12(1): p. e1005744
    [PMID:26745719]