PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G34370.2
Common NameAtSTOP1, F7P12.7, STOP1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family C2H2
Protein Properties Length: 499aa    MW: 55206.8 Da    PI: 6.1341
Description C2H2 family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G34370.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1zf-C2H221.18.3e-07245266223
                  EETTTTEEESSHHHHHHHHHHT CS
      zf-C2H2   2 kCpdCgksFsrksnLkrHirtH 23 
                   C++Cgk F+r  nL+ H+r H
  AT1G34370.2 245 FCTICGKGFKRDANLRMHMRGH 266
                  6*******************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF576672.17E-5242270No hitNo description
PROSITE profilePS5015712.03244271IPR007087Zinc finger, C2H2
SMARTSM003550.0026244266IPR015880Zinc finger, C2H2-like
Gene3DG3DSA:3.30.160.607.5E-6245297IPR013087Zinc finger C2H2-type/integrase DNA-binding domain
PROSITE patternPS000280246266IPR007087Zinc finger, C2H2
SMARTSM0035567295328IPR015880Zinc finger, C2H2-like
SMARTSM0035545333355IPR015880Zinc finger, C2H2-like
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0010044Biological Processresponse to aluminum ion
GO:0010447Biological Processresponse to acidic pH
GO:0005634Cellular Componentnucleus
GO:0003676Molecular Functionnucleic acid binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0008270Molecular Functionzinc ion binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 499 aa     Download sequence    Send to blast
METEDDLCNT NWGSSSSKSR EPGSSDCGNS TFAGFTSQQK WEDASILDYE MGVEPGLQES  60
IQANVDFLQG VRAQAWDPRT MLSNLSFMEQ KIHQLQDLVH LLVGRGGQLQ GRQDELAAQQ  120
QQLITTDLTS IIIQLISTAG SLLPSVKHNM STAPGPFTGQ PGSAVFPYVR EANNVASQSQ  180
NNNNCGAREF DLPKPVLVDE REGHVVEEHE MKDEDDVEEG ENLPPGSYEI LQLEKEEILA  240
PHTHFCTICG KGFKRDANLR MHMRGHGDEY KTAAALAKPN KESVPGSEPM LIKRYSCPFL  300
GCKRNKEHKK FQPLKTILCV KNHYKRTHCD KSFTCSRCHT KKFSVIADLK THEKHCGKNK  360
WLCSCGTTFS RKDKLFGHIA LFQGHTPAIP LEETKPSAST STQRGSSEGG NNNQGMVGFN  420
LGSASNANQE TTQPGMTDGR ICFEESFSPM NFDTCNFGGF HEFPRLMFDD SESSFQMLIA  480
NACGFSPRNV GESVSDTSL
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.640180.0root| seed| silique| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible259932_at0.0
Expression AtlasAT1G34370-
AtGenExpressAT1G34370-
ATTED-IIAT1G34370-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots (e.g. root tips and lateral roots), leaves, flowers (e.g. stigma, sepal, anther, and filament), stems, siliques and cotyledons. {ECO:0000269|PubMed:23935008}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a putative nuclear Cys(2)His(2)-type zinc finger protein involved in H+ and Al3+ rhizotoxicity. In mutants exposed to aluminum stress, there is no induction of AtALMT1, an malate transporter known to be involved in the mediation of aluminum toxicity.
UniProtProbable transcription factor. Together with STOP2, plays a critical role in tolerance to major stress factors in acid soils such as proton H(+) and aluminum ion Al(3+). Required for the expression of genes in response to acidic stress (e.g. ALMT1 and MATE), and Al-activated citrate exudation. {ECO:0000269|PubMed:17535918, ECO:0000269|PubMed:18826429, ECO:0000269|PubMed:19321711, ECO:0000269|PubMed:23935008}.
Function -- GeneRIF ? help Back to Top
  1. STOP1 plays a critical role in Arabidopsis tolerance to major stress factors in acid soils. [STOP1]
    [PMID: 17535918]
  2. STOP1 is localized to the nucleus and regulates the expression of multiple genes that protect Arabidopsis from Al and H(+) toxicities.
    [PMID: 19321711]
  3. STOP2, a STOP1 homolog, partially recovered Al- and low pH-tolerance by recovering the expression of genes regulated by STOP1.
    [PMID: 23935008]
  4. Mutation in a position around -297 from the first ATG completely inactivated AtALMT1 expression and Al response that contained the STOP1 binding site.
    [PMID: 25627216]
  5. Results indicate STOP1 and ALMT1 as a signalling pathway of low phosphate (Pi) availability and exuded malate as an unexpected apoplastic inhibitor of root cell wall expansion.
    [PMID: 28504266]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00196ampDAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G34370.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By shock H(+) and Al(3+) treatments. {ECO:0000269|PubMed:17535918}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G08430(A), AT3G22200(A), AT5G07440(A)
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9C8N5
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Hypersensitive to H(+) and Al(3+) rhizotoxicity, reduced induction of genes such as ALMT1 and MATE in response to acidic stress, and impaired Al-activated citrate exudation. {ECO:0000269|PubMed:17535918, ECO:0000269|PubMed:18826429, ECO:0000269|PubMed:19321711}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G34370
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB3002360.0AB300236.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Ler.
GenBankAB3002380.0AB300238.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Kl-5.
GenBankAB3002390.0AB300239.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Col-4.
GenBankAB3002400.0AB300240.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Tu-0.
GenBankAB3002410.0AB300241.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Lo-1.
GenBankAB3002420.0AB300242.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Kb-0.
GenBankAB3002430.0AB300243.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Pi-0.
GenBankAB3002440.0AB300244.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Fr-3.
GenBankAB3002450.0AB300245.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Go-0.
GenBankAB3002460.0AB300246.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Van-3.
GenBankAB3002470.0AB300247.1 Arabidopsis thaliana STOP1 gene for C2-H2 zinc finger protein, complete cds, ecotype: Li-1.
GenBankAC0239130.0AC023913.7 Arabidopsis thaliana chromosome 1 BAC F7P12 genomic sequence, complete sequence.
GenBankAK2271280.0AK227128.1 Arabidopsis thaliana mRNA for putative zinc finger protein, complete cds, clone: RAFL09-80-D13.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_174697.10.0C2H2 and C2HC zinc fingers superfamily protein
RefseqNP_849746.10.0C2H2 and C2HC zinc fingers superfamily protein
SwissprotQ9C8N50.0STOP1_ARATH; Protein SENSITIVE TO PROTON RHIZOTOXICITY 1
TrEMBLA0A178WM470.0A0A178WM47_ARATH; STOP1
STRINGAT1G34370.20.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Englbrecht CC,Schoof H,B
    Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome.
    BMC Genomics, 2004. 5(1): p. 39
    [PMID:15236668]
  3. Iuchi S, et al.
    Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance.
    Proc. Natl. Acad. Sci. U.S.A., 2007. 104(23): p. 9900-5
    [PMID:17535918]
  4. Soitamo AJ,Piippo M,Allahverdiyeva Y,Battchikova N,Aro EM
    Light has a specific role in modulating Arabidopsis gene expression at low temperature.
    BMC Plant Biol., 2008. 8: p. 13
    [PMID:18230142]
  5. Dortay H, et al.
    Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana.
    J. Proteome Res., 2008. 7(9): p. 3649-60
    [PMID:18642946]
  6. Houde M,Diallo AO
    Identification of genes and pathways associated with aluminum stress and tolerance using transcriptome profiling of wheat near-isogenic lines.
    BMC Genomics, 2008. 9: p. 400
    [PMID:18752686]
  7. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  8. Liu J,Magalhaes JV,Shaff J,Kochian LV
    Aluminum-activated citrate and malate transporters from the MATE and ALMT families function independently to confer Arabidopsis aluminum tolerance.
    Plant J., 2009. 57(3): p. 389-99
    [PMID:18826429]
  9. Sawaki Y, et al.
    STOP1 regulates multiple genes that protect arabidopsis from proton and aluminum toxicities.
    Plant Physiol., 2009. 150(1): p. 281-94
    [PMID:19321711]
  10. Iuchi S,Kobayashi Y,Koyama H,Kobayashi M
    STOP1, a Cys2/His2 type zinc-finger protein, plays critical role in acid soil tolerance in Arabidopsis.
    Plant Signal Behav, 2008. 3(2): p. 128-30
    [PMID:19704731]
  11. Zhou C,Yin Y,Dam P,Xu Y
    Identification of novel proteins involved in plant cell-wall synthesis based on protein-protein interaction data.
    J. Proteome Res., 2010. 9(10): p. 5025-37
    [PMID:20687615]
  12. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  13. Ohyama Y, et al.
    Characterization of AtSTOP1 orthologous genes in tobacco and other plant species.
    Plant Physiol., 2013. 162(4): p. 1937-46
    [PMID:23749850]
  14. Kobayashi Y, et al.
    Molecular and physiological analysis of Al³⁺ and H⁺ rhizotoxicities at moderately acidic conditions.
    Plant Physiol., 2013. 163(1): p. 180-92
    [PMID:23839867]
  15. Kobayashi Y, et al.
    STOP2 activates transcription of several genes for Al- and low pH-tolerance that are regulated by STOP1 in Arabidopsis.
    Mol Plant, 2014. 7(2): p. 311-22
    [PMID:23935008]
  16. Yokosho K,Yamaji N,Ma JF
    Global transcriptome analysis of Al-induced genes in an Al-accumulating species, common buckwheat (Fagopyrum esculentum Moench).
    Plant Cell Physiol., 2014. 55(12): p. 2077-91
    [PMID:25273892]
  17. Tokizawa M, et al.
    SENSITIVE TO PROTON RHIZOTOXICITY1, CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2, and other transcription factors are involved in ALUMINUM-ACTIVATED MALATE TRANSPORTER1 expression.
    Plant Physiol., 2015. 167(3): p. 991-1003
    [PMID:25627216]
  18. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  19. Geng X, et al.
    LEUNIG_HOMOLOG transcriptional co-repressor mediates aluminium sensitivity through PECTIN METHYLESTERASE46-modulated root cell wall pectin methylesterification in Arabidopsis.
    Plant J., 2017. 90(3): p. 491-504
    [PMID:28181322]
  20. Balzergue C, et al.
    Low phosphate activates STOP1-ALMT1 to rapidly inhibit root cell elongation.
    Nat Commun, 2017. 8: p. 15300
    [PMID:28504266]
  21. Pelagio-Flores R,Esparza-Reynoso S,Garnica-Vergara A,López-Bucio J,Herrera-Estrella A
    Trichoderma-Induced Acidification Is an Early Trigger for Changes in Arabidopsis Root Growth and Determines Fungal Phytostimulation.
    Front Plant Sci, 2017. 8: p. 822
    [PMID:28567051]
  22. Jiang F, et al.
    Identification and characterization of suppressor mutants of stop1.
    BMC Plant Biol., 2017. 17(1): p. 128
    [PMID:28738784]
  23. Daspute AA, et al.
    Transcriptional Regulation of Aluminum-Tolerance Genes in Higher Plants: Clarifying the Underlying Molecular Mechanisms.
    Front Plant Sci, 2017. 8: p. 1358
    [PMID:28848571]
  24. Sharma A,Wai CM,Ming R,Yu Q
    Diurnal Cycling Transcription Factors of Pineapple Revealed by Genome-Wide Annotation and Global Transcriptomic Analysis.
    Genome Biol Evol, 2017. 9(9): p. 2170-2190
    [PMID:28922793]
  25. Zhang Y, et al.
    The Cell Cycle Checkpoint Regulator ATR Is Required for Internal Aluminum Toxicity-Mediated Root Growth Inhibition in Arabidopsis.
    Front Plant Sci, 2018. 9: p. 118
    [PMID:29491872]