PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G32330.1
Common NameATHSFA1D, F27G20.6, F5D14.8, HSF01, HSF8, HSFA1D
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HSF
Protein Properties Length: 485aa    MW: 54524.4 Da    PI: 4.6383
Description heat shock transcription factor A1D
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G32330.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HSF_DNA-bind113.51.4e-35381292102
                   HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS
  HSF_DNA-bind   2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 
                   Fl+k+y++++d+++++++sws+n+nsf+v+++ efa+++Lpk Fkh+nf+SFvRQLn+YgF+kv+ ++         weF+++ F +g+k+ll++i 
   AT1G32330.1  38 FLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQLNTYGFRKVDPDR---------WEFANEGFLRGQKHLLQSIT 125
                   9****************************************************************999.........******************** PP

                   XXXX CS
  HSF_DNA-bind  99 rkks 102
                   r+k 
   AT1G32330.1 126 RRKP 129
                   *986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.10.10.101.9E-3831121IPR011991Winged helix-turn-helix DNA-binding domain
SMARTSM004155.6E-6034127IPR000232Heat shock factor (HSF)-type, DNA-binding
SuperFamilySSF467851.36E-3434127IPR011991Winged helix-turn-helix DNA-binding domain
PRINTSPR000561.3E-183861IPR000232Heat shock factor (HSF)-type, DNA-binding
PfamPF004474.2E-3138127IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000561.3E-187688IPR000232Heat shock factor (HSF)-type, DNA-binding
PROSITE patternPS00434077101IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000561.3E-1889101IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009408Biological Processresponse to heat
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 485 aa     Download sequence    Send to blast
MDVSKVTTSD GGGDSMETKP SPQPQPAAIL SSNAPPPFLS KTYDMVDDHN TDSIVSWSAN  60
NNSFIVWKPP EFARDLLPKN FKHNNFSSFV RQLNTYGFRK VDPDRWEFAN EGFLRGQKHL  120
LQSITRRKPA HGQGQGHQRS QHSNGQNSSV SACVEVGKFG LEEEVERLKR DKNVLMQELV  180
RLRQQQQSTD NQLQTMVQRL QGMENRQQQL MSFLAKAVQS PHFLSQFLQQ QNQQNESNRR  240
ISDTSKKRRF KRDGIVRNND SATPDGQIVK YQPPMHEQAK AMFKQLMKME PYKTGDDGFL  300
LGNGTSTTEG TEMETSSNQV SGITLKEMPT ASEIQSSSPI ETTPENVSAA SEATENCIPS  360
PDDLTLPDFT HMLPENNSEK PPESFMEPNL GGSSPLLDPD LLIDDSLSFD IDDFPMDSDI  420
DPVDYGLLER LLMSSPVPDN MDSTPVDNET EQEQNGWDKT KHMDNLTQQM GLLSPETLDL  480
SRQNP
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5d5v_D1e-242612718129Heat shock factor protein 1
5d5v_B1e-242612718129Heat shock factor protein 1
5d5u_B1e-242612718129Heat shock factor protein 1
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1245250KKRRFK
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.482560.0flower| seed| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible260701_at0.0
Expression AtlasAT1G32330-
AtGenExpressAT1G32330-
ATTED-IIAT1G32330-
Functional Description ? help Back to Top
Source Description
TAIRMember of Heat Stress Transcription Factor (Hsf) family. Negatively regulated by HSP90.2.
UniProtTranscriptional regulator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
Function -- GeneRIF ? help Back to Top
  1. These findings indicated that HsfA1d and HsfA1e not only regulate HsfA2 expression but also function as key regulators of the Hsf signaling network in response to environmental stress.
    [PMID: 21471117]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G32330.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G26150(A)
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G32330
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAC0841100.0AC084110.4 Arabidopsis thaliana chromosome 1 BAC F27G20 genomic sequence, complete sequence.
GenBankF5D140.0AC007767.3 Sequence of BAC F5D14 from Arabidopsis thaliana chromosome 1, complete sequence.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_174511.20.0heat stress transcription factor A-1d
SwissprotQ9LQM70.0HFA1D_ARATH; Heat stress transcription factor A-1d
TrEMBLD7KHI80.0D7KHI8_ARALL; ATHSFA1D
STRINGAT1G32330.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP9617233
MalvidsOGEM64702440
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nover L, et al.
    Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?
    Cell Stress Chaperones, 2001. 6(3): p. 177-89
    [PMID:11599559]
  3. Seki M, et al.
    Functional annotation of a full-length Arabidopsis cDNA collection.
    Science, 2002. 296(5565): p. 141-5
    [PMID:11910074]
  4. Zhu H, et al.
    Syntenic relationships between Medicago truncatula and Arabidopsis reveal extensive divergence of genome organization.
    Plant Physiol., 2003. 131(3): p. 1018-26
    [PMID:12644654]
  5. Yamada K, et al.
    Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana.
    J. Biol. Chem., 2007. 282(52): p. 37794-804
    [PMID:17965410]
  6. Guo J, et al.
    Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis.
    J Genet Genomics, 2008. 35(2): p. 105-18
    [PMID:18407058]
  7. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  8. Yamada K,Nishimura M
    Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana.
    Plant Signal Behav, 2008. 3(9): p. 660-2
    [PMID:19704818]
  9. Xu X, et al.
    Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress.
    Biotechnol. Lett., 2010. 32(7): p. 979-87
    [PMID:20229063]
  10. Liu HC,Liao HT,Charng YY
    The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis.
    Plant Cell Environ., 2011. 34(5): p. 738-51
    [PMID:21241330]
  11. Nishizawa-Yokoi A, et al.
    HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the Hsf signaling network in response to environmental stress.
    Plant Cell Physiol., 2011. 52(5): p. 933-45
    [PMID:21471117]
  12. Yoshida T, et al.
    Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression.
    Mol. Genet. Genomics, 2011. 286(5-6): p. 321-32
    [PMID:21931939]
  13. Liu HC,Charng YY
    Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response.
    Plant Signal Behav, 2012. 7(5): p. 547-50
    [PMID:22516818]
  14. Liu J, et al.
    An autoregulatory loop controlling Arabidopsis HsfA2 expression: role of heat shock-induced alternative splicing.
    Plant Physiol., 2013. 162(1): p. 512-21
    [PMID:23503691]
  15. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]