PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G29280.1
Common NameATWRKY65, F28N24.4, WRKY65
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 259aa    MW: 29054.4 Da    PI: 5.4693
Description WRKY DNA-binding protein 65
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G29280.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY99.91.5e-3174132259
                  --SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                  +D ++WrKYGqK++kgs++pr+YYrC+s+ gCp++k+vers++dp+++ itY++eHnh+
  AT1G29280.1  74 SDSWAWRKYGQKPIKGSPYPRGYYRCSSTkGCPARKQVERSRDDPTMILITYTSEHNHP 132
                  69*************************998****************************7 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.802.0E-3265134IPR003657WRKY domain
PROSITE profilePS5081132.29468134IPR003657WRKY domain
SuperFamilySSF1182901.83E-2769134IPR003657WRKY domain
SMARTSM007745.6E-3873133IPR003657WRKY domain
PfamPF031061.2E-2675132IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009025anatomyvascular leaf
PO:0009031anatomysepal
PO:0009047anatomystem
PO:0020030anatomycotyledon
PO:0020100anatomyhypocotyl
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 259 aa     Download sequence    Send to blast
MKRGLDMARS YNDHESSQET GPESPNSSTF NGMKALISSH SPKRSRRSVE KRVVNVPMKE  60
MEGSRHKGDT TPPSDSWAWR KYGQKPIKGS PYPRGYYRCS STKGCPARKQ VERSRDDPTM  120
ILITYTSEHN HPWPLTSSTR NGPKPKPEPK PEPEPEVEPE AEEEDNKFMV LGRGIETTPS  180
CVDEFAWFTE METTSSTILE SPIFSSEKKT AVSGADDVAV FFPMGEEDES LFADLGELPE  240
CSVVFRHRSS VVGSQVEIF
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5w3x_B2e-2151134679Disease resistance protein RRS1
5w3x_D2e-2151134679Disease resistance protein RRS1
Search in ModeBase
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible260882_at0.0
Expression AtlasAT1G29280-
AtGenExpressAT1G29280-
ATTED-IIAT1G29280-
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group II-e
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00167DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G29280.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G29280
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4521730.0AF452173.1 Arabidopsis thaliana WRKY transcription factor 65 (WRKY65) mRNA, complete cds.
GenBankBT0263540.0BT026354.1 Arabidopsis thaliana At1g29280 gene, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_174222.20.0WRKY DNA-binding protein 65
SwissprotQ9LP560.0WRK65_ARATH; Probable WRKY transcription factor 65
TrEMBLA0A384LK500.0A0A384LK50_ARATH; WRKY65
TrEMBLQ0V8660.0Q0V866_ARATH; At1g29280
STRINGAT1G29280.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM53832849
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Dal Bosco C, et al.
    Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana.
    J. Biol. Chem., 2004. 279(2): p. 1060-9
    [PMID:14576160]
  4. Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK
    Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.
    Plant J., 2004. 38(2): p. 366-79
    [PMID:15078338]
  5. Gong W, et al.
    Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes.
    Plant Physiol., 2004. 135(2): p. 773-82
    [PMID:15208423]
  6. Contento AL,Kim SJ,Bassham DC
    Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation.
    Plant Physiol., 2004. 135(4): p. 2330-47
    [PMID:15310832]
  7. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  8. Pischke MS,Huttlin EL,Hegeman AD,Sussman MR
    A transcriptome-based characterization of habituation in plant tissue culture.
    Plant Physiol., 2006. 140(4): p. 1255-78
    [PMID:16489130]
  9. Singh V,Roy S,Singh D,Nandi AK
    Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes.
    J. Biosci., 2014. 39(1): p. 119-26
    [PMID:24499796]