PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G27740.1
Common NameBHLH54, EN114, RSL4, T22C5.19
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 258aa    MW: 29159.8 Da    PI: 6.6492
Description root hair defective 6-like 4
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G27740.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH34.34.3e-111832231055
                  HHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH  10 rrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  r+RR++iN+++ +L+ l+P+      +K++ +++Le+Av+Y+k Lq
  AT1G27740.1 183 RKRREKINERLKTLQNLVPNG-----TKVDISTMLEEAVHYVKFLQ 223
                  9******************97.....8******************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088815.123173222IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474592.09E-14175237IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.1E-13178232IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.0E-12179228IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000837.62E-12182227No hitNo description
PfamPF000101.9E-8183223IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009733Biological Processresponse to auxin
GO:0016049Biological Processcell growth
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0045944Biological Processpositive regulation of transcription from RNA polymerase II promoter
GO:0048766Biological Processroot hair initiation
GO:0005634Cellular Componentnucleus
GO:0001046Molecular Functioncore promoter sequence-specific DNA binding
GO:0001228Molecular Functiontranscriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000256anatomyroot hair cell
PO:0000262anatomytrichoblast
PO:0009005anatomyroot
PO:0007519developmental stage5 root hair formation stage
Sequence ? help Back to Top
Protein Sequence    Length: 258 aa     Download sequence    Send to blast
MDVFVDGELE SLLGMFNFDQ CSSSKEERPR DELLGLSSLY NGHLHQHQHH NNVLSSDHHA  60
FLLPDMFPFG AMPGGNLPAM LDSWDQSHHL QETSSLKRKL LDVENLCKTN SNCDVTRQEL  120
AKSKKKQRVS SESNTVDESN TNWVDGQSLS NSSDDEKASV TSVKGKTRAT KGTATDPQSL  180
YARKRREKIN ERLKTLQNLV PNGTKVDIST MLEEAVHYVK FLQLQIKLLS SDDLWMYAPL  240
AYNGLDMGFH HNLLSRLM
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1120126AKSKKKQ
2182187RKRREK
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453361790.0
Genevisible261647_at0.0
Expression AtlasAT1G27740-
AtGenExpressAT1G27740-
ATTED-IIAT1G27740-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and flowers. {ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
TAIRBasic helix-loop-helix (bHLH) transcription factor that is sufficient to promote postmitotic cell growth in root-hair cells. RSL4 is a direct transcriptional target of RHD6
Function -- GeneRIF ? help Back to Top
  1. study reports the discovery of a basic helix-loop-helix (bHLH) transcription factor called RSL4 (ROOT HAIR DEFECTIVE 6-LIKE 4) that is sufficient to promote postmitotic cell growth in Arabidopsis thaliana root-hair cells
    [PMID: 20139979]
  2. RSL4 controls cell growth by controlling the expression of genes encoding proteins involved in cell signalling, cell wall modification and secretion.
    [PMID: 27452638]
  3. Results suggest that RSL4 mediates root hair formation by regulating root hair-specific cis-element (RHE) genes and that this mechanism is conserved throughout the tracheophyte (vascular plant) lineage.
    [PMID: 28087829]
  4. A trihelix transcription factor GT-2-LIKE1 (GTL1) and its homolog DF1 repress root hair growth. Transcriptional data, combined with genome-wide chromatin-binding data, show that GTL1 and DF1 directly bind the RSL4 promoter and regulate its expression to repress root hair growth.
    [PMID: 29439132]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G27740.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G27740
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0854360.0AY085436.1 Arabidopsis thaliana clone 151587 mRNA, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_564293.10.0root hair defective 6-like 4
SwissprotQ8LEG10.0BH054_ARATH; Transcription factor bHLH54
TrEMBLA0A384L1Z00.0A0A384L1Z0_ARATH; RSL4
TrEMBLC0SUX80.0C0SUX8_ARATH; Uncharacterized protein At1g27740 (Fragment)
STRINGAT1G27740.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM41322257
Representative plantOGRP10481454
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Abe H, et al.
    Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell, 2003. 15(1): p. 63-78
    [PMID:12509522]
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  5. Yi K,Menand B,Bell E,Dolan L
    A basic helix-loop-helix transcription factor controls cell growth and size in root hairs.
    Nat. Genet., 2010. 42(3): p. 264-7
    [PMID:20139979]
  6. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  7. Bruex A, et al.
    A gene regulatory network for root epidermis cell differentiation in Arabidopsis.
    PLoS Genet., 2012. 8(1): p. e1002446
    [PMID:22253603]
  8. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  9. Han Y, et al.
    Altered expression of TaRSL4 gene by genome interplay shapes root hair length in allopolyploid wheat.
    New Phytol., 2016. 209(2): p. 721-32
    [PMID:26334764]
  10. Datta S,Prescott H,Dolan L
    Intensity of a pulse of RSL4 transcription factor synthesis determines Arabidopsis root hair cell size.
    Nat Plants, 2015. 1: p. 15138
    [PMID:27251390]
  11. Song L, et al.
    The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation.
    PLoS Genet., 2016. 12(7): p. e1006194
    [PMID:27427911]
  12. Vijayakumar P,Datta S,Dolan L
    ROOT HAIR DEFECTIVE SIX-LIKE4 (RSL4) promotes root hair elongation by transcriptionally regulating the expression of genes required for cell growth.
    New Phytol., 2016. 212(4): p. 944-953
    [PMID:27452638]
  13. Hwang Y,Choi HS,Cho HM,Cho HT
    Tracheophytes Contain Conserved Orthologs of a Basic Helix-Loop-Helix Transcription Factor That Modulate ROOT HAIR SPECIFIC Genes.
    Plant Cell, 2017. 29(1): p. 39-53
    [PMID:28087829]
  14. Marzol E,Borassi C,Denita Juárez SP,Mangano S,Estevez JM
    RSL4 Takes Control: Multiple Signals, One Transcription Factor.
    Trends Plant Sci., 2017. 22(7): p. 553-555
    [PMID:28487046]
  15. Stanley CE, et al.
    Dual-flow-RootChip reveals local adaptations of roots towards environmental asymmetry at the physiological and genetic levels.
    New Phytol., 2018. 217(3): p. 1357-1369
    [PMID:29125191]
  16. Feng Y, et al.
    Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2017. 114(52): p. 13834-13839
    [PMID:29233944]
  17. Shibata M, et al.
    GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis.
    Development, 2018.
    [PMID:29439132]