PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G25330.1
Common NameBHLH75, CES, EN78, F4F7.28, HAF
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 223aa    MW: 25209.3 Da    PI: 6.9554
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G25330.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH26.71e-08114161455
                  HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  +h+ +Er RR++iN+++  L++l+P +     k +  a +L   ++Y++sLq
  AT1G25330.1 114 SHSLAERVRREKINERLKCLQDLVPGC----YKAMGMAVMLDVIIDYVRSLQ 161
                  8*************************8....345888999999999999998 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474591.57E-16108177IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.15E-11108165No hitNo description
PROSITE profilePS5088815.605110160IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.8E-16110172IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000104.8E-6114161IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003535.1E-9116166IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:2000488Biological Processpositive regulation of brassinosteroid biosynthetic process
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009009anatomyplant embryo
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009052anatomyflower pedicel
PO:0020094anatomyplant egg cell
PO:0025022anatomycollective leaf structure
PO:0001185developmental stageplant embryo globular stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 223 aa     Download sequence    Send to blast
MARFEPYNYN NGHDPFFAHI NQNPELINLD LPASTPSSFM LFSNGALVDA NHNNSHFFPN  60
LLHGNTRRKG NKEESGSKRR RKRSEEEEAM NGDETQKPKD VVHVRAKRGQ ATDSHSLAER  120
VRREKINERL KCLQDLVPGC YKAMGMAVML DVIIDYVRSL QNQIEFLSMK LSAASACYDL  180
NSLDIEPTDI FQGGNIHSAA EMERILRESV GTQPPNFSST LPF
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
16682RRKGNKEESGSKRRRKR
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.639800.0flower
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1864788670.0
Genevisible245640_at0.0
Expression AtlasAT1G25330-
AtGenExpressAT1G25330-
ATTED-IIAT1G25330-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in leaves, stems, and flowers. {ECO:0000269|PubMed:12679534}.
Function -- GeneRIF ? help Back to Top
  1. HALF FILLED (HAF) gene, a bHLH-encoding transcription factor, is required for reproductive tract development. HAF acts redundantly with two closely related genes, BRASSINOSTEROID ENHANCED EXPRESSION1 (BEE1) and BEE3.
    [PMID: 21693516]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G25330.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
IntActSearch A4D998
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G25330
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankDQ4462890.0DQ446289.1 Arabidopsis thaliana clone pENTR221-At1g25330 basic helix-loop-helix family protein (At1g25330) mRNA, complete cds.
GenBankAY0888850.0AY088885.1 Arabidopsis thaliana clone 98716 mRNA, complete sequence.
GenBankAK1757570.0AK175757.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor (bHLH075), complete cds, clone: RAFL22-31-M12.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_564229.11e-168transcription factor CESTA
SwissprotA4D9981e-170BH075_ARATH; Transcription factor bHLH75
TrEMBLA0A0D3BI541e-136A0A0D3BI54_BRAOL; Uncharacterized protein
STRINGAT1G25330.11e-167(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP5816313
MalvidsOGEM140812024
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Friedrichsen DM, et al.
    Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth.
    Genetics, 2002. 162(3): p. 1445-56
    [PMID:12454087]
  3. Abe H, et al.
    Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell, 2003. 15(1): p. 63-78
    [PMID:12509522]
  4. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  5. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  6. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  7. Cao D,Cheng H,Wu W,Soo HM,Peng J
    Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis.
    Plant Physiol., 2006. 142(2): p. 509-25
    [PMID:16920880]
  8. Underwood BA,Vanderhaeghen R,Whitford R,Town CD,Hilson P
    Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations.
    Plant Biotechnol. J., 2006. 4(3): p. 317-24
    [PMID:17147637]
  9. Gong W, et al.
    The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors.
    Mol Plant, 2008. 1(1): p. 27-41
    [PMID:19802365]
  10. Wuest SE, et al.
    Arabidopsis female gametophyte gene expression map reveals similarities between plant and animal gametes.
    Curr. Biol., 2010. 20(6): p. 506-12
    [PMID:20226671]
  11. Poppenberger B, et al.
    CESTA, a positive regulator of brassinosteroid biosynthesis.
    EMBO J., 2011. 30(6): p. 1149-61
    [PMID:21336258]
  12. Crawford BC,Yanofsky MF
    HALF FILLED promotes reproductive tract development and fertilization efficiency in Arabidopsis thaliana.
    Development, 2011. 138(14): p. 2999-3009
    [PMID:21693516]
  13. Meinke DW
    A survey of dominant mutations in Arabidopsis thaliana.
    Trends Plant Sci., 2013. 18(2): p. 84-91
    [PMID:22995285]