PlantTFDB
Plant Transcription Factor Database
PlantRegMap/PlantTFDB v5.0
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G22070.1
Common NameBZIP22, F2E2.14, TGA3
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 384aa    MW: 43755 Da    PI: 6.1266
Description TGA1A-related gene 3
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G22070.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1bZIP_129.91.3e-0997137444
                  XCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS
       bZIP_1   4 lkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNka 44 
                  +k  rr+++NReAAr+sR+RKka +++Lee   +L++  ++
  AT1G22070.1  97 DKMKRRLAQNREAARKSRLRKKAHVQQLEESRLKLSQLEQE 137
                  7899*************************987777766555 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.20.5.1701.9E-788138No hitNo description
SMARTSM003380.002490152IPR004827Basic-leucine zipper domain
PROSITE profilePS502179.34796138IPR004827Basic-leucine zipper domain
SuperFamilySSF579593.21E-698140No hitNo description
PfamPF077162.3E-598143IPR004827Basic-leucine zipper domain
PROSITE patternPS000360101116IPR004827Basic-leucine zipper domain
PfamPF141449.5E-29185259IPR025422Transcription factor TGA like domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009626Biological Processplant-type hypersensitive response
GO:0009862Biological Processsystemic acquired resistance, salicylic acid mediated signaling pathway
GO:0042742Biological Processdefense response to bacterium
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005516Molecular Functioncalmodulin binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 384 aa     Download sequence    Send to blast
MEMMSSSSST TQVVSFRDMG MYEPFQQLSG WESPFKSDIN NITSNQNNNQ SSSTTLEVDA  60
RPEADDNNRV NYTSVYNNSL EAEPSSNNDQ DEDRINDKMK RRLAQNREAA RKSRLRKKAH  120
VQQLEESRLK LSQLEQELVR ARQQGLCVRN SSDTSYLGPA GNMNSGIAAF EMEYTHWLEE  180
QNRRVSEIRT ALQAHIGDIE LKMLVDSCLN HYANLFRMKA DAAKADVFFL MSGMWRTSTE  240
RFFQWIGGFR PSELLNVVMP YVEPLTDQQL LEVRNLQQSS QQAEEALSQG LDKLQQGLVE  300
SIAIQIKVVE SVNHGAPMAS AMENLQALES FVNQADHLRQ QTLQQMSKIL TTRQAARGLL  360
ALGEYFHRLR ALSSLWAARP REHT
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.156140.0flower| leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible255953_at0.0
Expression AtlasAT1G22070-
AtGenExpressAT1G22070-
ATTED-IIAT1G22070-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in the whole plant. {ECO:0000269|PubMed:8003690, ECO:0000269|PubMed:8628224}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a transcription factor. Like other TGAla-related factors, TGA3 has a highly conserved bZIP region and exhibits similar DNA-binding properties.
UniProtTranscriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Required to induce the systemic acquired resistance (SAR) via the regulation of pathogenesis-related genes expression. Binding to the as-1 element of PR-1 promoter is salicylic acid-inducible and mediated by NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters. {ECO:0000269|PubMed:12897257}.
Function -- GeneRIF ? help Back to Top
  1. Results show that cytokinins modulate salicylic acid signaling to augment resistance against Pst, a process in which the interaction between NPR1/TGA3 and ARR2 is important.
    [PMID: 20708590]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00157DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G22070.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G14610(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDsalicylic acid
Interaction ? help Back to Top
Source Intact With
IntActSearch Q39234
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G22070
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankATHTAGIA0.0L10209.1 Arabidopsis thaliana transcription factor mRNA, complete cds.
GenBankBT0260340.0BT026034.1 Arabidopsis thaliana At1g22070 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_564156.10.0transcription factor TGA3
SwissprotQ392340.0TGA3_ARATH; Transcription factor TGA3
TrEMBLQ147Q90.0Q147Q9_ARATH; At1g22070
STRINGAT1G22070.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM30612766
Representative plantOGRP26791132
Publications ? help Back to Top
  1. van Der Luit AH,Olivari C,Haley A,Knight MR,Trewavas AJ
    Distinct calcium signaling pathways regulate calmodulin gene expression in tobacco.
    Plant Physiol., 1999. 121(3): p. 705-14
    [PMID:10557218]
  2. Zhou JM, et al.
    NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid.
    Mol. Plant Microbe Interact., 2000. 13(2): p. 191-202
    [PMID:10659709]
  3. Després C,DeLong C,Glaze S,Liu E,Fobert PR
    The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors.
    Plant Cell, 2000. 12(2): p. 279-90
    [PMID:10662863]
  4. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  5. Chern MS, et al.
    Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis.
    Plant J., 2001. 27(2): p. 101-13
    [PMID:11489188]
  6. Reddy VS,Ali GS,Reddy AS
    Genes encoding calmodulin-binding proteins in the Arabidopsis genome.
    J. Biol. Chem., 2002. 277(12): p. 9840-52
    [PMID:11782485]
  7. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
    [PMID:11906833]
  8. Pandey S, et al.
    A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter.
    Eur. J. Biochem., 2002. 269(13): p. 3193-204
    [PMID:12084059]
  9. Johnson C,Boden E,Arias J
    Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis.
    Plant Cell, 2003. 15(8): p. 1846-58
    [PMID:12897257]
  10. Liu G,Holub EB,Alonso JM,Ecker JR,Fobert PR
    An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.
    Plant J., 2005. 41(2): p. 304-18
    [PMID:15634206]
  11. Hepworth SR,Zhang Y,McKim S,Li X,Haughn GW
    BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis.
    Plant Cell, 2005. 17(5): p. 1434-48
    [PMID:15805484]
  12. Kang HG,Klessig DF
    Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2.
    Plant Mol. Biol., 2005. 57(4): p. 541-57
    [PMID:15821979]
  13. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  14. Thibaud-Nissen F, et al.
    Development of Arabidopsis whole-genome microarrays and their application to the discovery of binding sites for the TGA2 transcription factor in salicylic acid-treated plants.
    Plant J., 2006. 47(1): p. 152-62
    [PMID:16824183]
  15. Zhang Y, et al.
    Negative regulation of defense responses in Arabidopsis by two NPR1 paralogs.
    Plant J., 2006. 48(5): p. 647-56
    [PMID:17076807]
  16. Kesarwani M,Yoo J,Dong X
    Genetic interactions of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis.
    Plant Physiol., 2007. 144(1): p. 336-46
    [PMID:17369431]
  17. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  18. Li S, et al.
    Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana.
    Plant Cell, 2009. 21(2): p. 429-41
    [PMID:19218396]
  19. Farinati S,DalCorso G,Varotto S,Furini A
    The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants.
    New Phytol., 2010. 185(4): p. 964-78
    [PMID:20028476]
  20. Choi J, et al.
    The cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis.
    Dev. Cell, 2010. 19(2): p. 284-95
    [PMID:20708590]
  21. Li S,Gutsche N,Zachgo S
    The ROXY1 C-terminal L**LL motif is essential for the interaction with TGA transcription factors.
    Plant Physiol., 2011. 157(4): p. 2056-68
    [PMID:21960138]
  22. Windram O, et al.
    Arabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis.
    Plant Cell, 2012. 24(9): p. 3530-57
    [PMID:23023172]
  23. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  24. Gangadharan A,Sreerekha MV,Whitehill J,Ham JH,Mackey D
    The Pseudomonas syringae pv. tomato type III effector HopM1 suppresses Arabidopsis defenses independent of suppressing salicylic acid signaling and of targeting AtMIN7.
    PLoS ONE, 2013. 8(12): p. e82032
    [PMID:24324742]
  25. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  26. Herrera-Vásquez A, et al.
    Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis.
    Plant Mol. Biol. Rep., 2018.
    [PMID:26696694]
  27. Fang H, et al.
    The Ca2+ /calmodulin2-binding transcription factor TGA3 elevates LCD expression and H2 S production to bolster Cr6+ tolerance in Arabidopsis.
    Plant J., 2017. 91(6): p. 1038-1050
    [PMID:28670772]
  28. Sarkar S, et al.
    Interaction of Arabidopsis TGA3 and WRKY53 transcription factors on Cestrum yellow leaf curling virus (CmYLCV) promoter mediates salicylic acid-dependent gene expression in planta.
    Planta, 2018. 247(1): p. 181-199
    [PMID:28913593]
  29. Lam E,Lam YK
    Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity.
    Nucleic Acids Res., 1995. 23(18): p. 3778-85
    [PMID:7479010]
  30. Miao ZH,Lam E
    Targeted disruption of the TGA3 locus in Arabidopsis thaliana.
    Plant J., 1995. 7(2): p. 359-65
    [PMID:7704051]
  31. Miao ZH,Liu X,Lam E
    TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.
    Plant Mol. Biol., 1994. 25(1): p. 1-11
    [PMID:8003690]
  32. de Pater S,Pham K,Memelink J,Kijne J
    Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2.
    Mol. Gen. Genet., 1996. 250(2): p. 237-9
    [PMID:8628224]
  33. Szymanski DB,Liao B,Zielinski RE
    Calmodulin isoforms differentially enhance the binding of cauliflower nuclear proteins and recombinant TGA3 to a region derived from the Arabidopsis Cam-3 promoter.
    Plant Cell, 1996. 8(6): p. 1069-77
    [PMID:8672891]