PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G22070.1
Common NameBZIP22, F2E2.14, TGA3
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 384aa    MW: 43755 Da    PI: 6.1266
Description TGA1A-related gene 3
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G22070.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
       bZIP_1   4 lkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNka 44 
                  +k  rr+++NReAAr+sR+RKka +++Lee   +L++  ++
                  7899*************************987777766555 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA: hitNo description
SMARTSM003380.002490152IPR004827Basic-leucine zipper domain
PROSITE profilePS502179.34796138IPR004827Basic-leucine zipper domain
SuperFamilySSF579593.21E-698140No hitNo description
PfamPF077162.3E-598143IPR004827Basic-leucine zipper domain
PROSITE patternPS000360101116IPR004827Basic-leucine zipper domain
PfamPF141449.5E-29185259IPR025422Transcription factor TGA like domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009626Biological Processplant-type hypersensitive response
GO:0009862Biological Processsystemic acquired resistance, salicylic acid mediated signaling pathway
GO:0042742Biological Processdefense response to bacterium
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005516Molecular Functioncalmodulin binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 384 aa     Download sequence    Send to blast
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.156140.0flower| leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT1G22070-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in the whole plant. {ECO:0000269|PubMed:8003690, ECO:0000269|PubMed:8628224}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a transcription factor. Like other TGAla-related factors, TGA3 has a highly conserved bZIP region and exhibits similar DNA-binding properties.
UniProtTranscriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Required to induce the systemic acquired resistance (SAR) via the regulation of pathogenesis-related genes expression. Binding to the as-1 element of PR-1 promoter is salicylic acid-inducible and mediated by NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters. {ECO:0000269|PubMed:12897257}.
Function -- GeneRIF ? help Back to Top
  1. Results show that cytokinins modulate salicylic acid signaling to augment resistance against Pst, a process in which the interaction between NPR1/TGA3 and ARR2 is important.
    [PMID: 20708590]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G14610(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDsalicylic acid
Interaction ? help Back to Top
Source Intact With
IntActSearch Q39234
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G22070
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankATHTAGIA0.0L10209.1 Arabidopsis thaliana transcription factor mRNA, complete cds.
GenBankBT0260340.0BT026034.1 Arabidopsis thaliana At1g22070 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_564156.10.0transcription factor TGA3
SwissprotQ392340.0TGA3_ARATH; Transcription factor TGA3
TrEMBLQ147Q90.0Q147Q9_ARATH; At1g22070
STRINGAT1G22070.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP26791132
Publications ? help Back to Top
  1. van Der Luit AH,Olivari C,Haley A,Knight MR,Trewavas AJ
    Distinct calcium signaling pathways regulate calmodulin gene expression in tobacco.
    Plant Physiol., 1999. 121(3): p. 705-14
  2. Zhou JM, et al.
    NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid.
    Mol. Plant Microbe Interact., 2000. 13(2): p. 191-202
  3. Després C,DeLong C,Glaze S,Liu E,Fobert PR
    The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors.
    Plant Cell, 2000. 12(2): p. 279-90
  4. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  5. Chern MS, et al.
    Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis.
    Plant J., 2001. 27(2): p. 101-13
  6. Reddy VS,Ali GS,Reddy AS
    Genes encoding calmodulin-binding proteins in the Arabidopsis genome.
    J. Biol. Chem., 2002. 277(12): p. 9840-52
  7. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
  8. Pandey S, et al.
    A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter.
    Eur. J. Biochem., 2002. 269(13): p. 3193-204
  9. Johnson C,Boden E,Arias J
    Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis.
    Plant Cell, 2003. 15(8): p. 1846-58
  10. Liu G,Holub EB,Alonso JM,Ecker JR,Fobert PR
    An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.
    Plant J., 2005. 41(2): p. 304-18
  11. Hepworth SR,Zhang Y,McKim S,Li X,Haughn GW
    BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis.
    Plant Cell, 2005. 17(5): p. 1434-48
  12. Kang HG,Klessig DF
    Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2.
    Plant Mol. Biol., 2005. 57(4): p. 541-57
  13. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
  14. Thibaud-Nissen F, et al.
    Development of Arabidopsis whole-genome microarrays and their application to the discovery of binding sites for the TGA2 transcription factor in salicylic acid-treated plants.
    Plant J., 2006. 47(1): p. 152-62
  15. Zhang Y, et al.
    Negative regulation of defense responses in Arabidopsis by two NPR1 paralogs.
    Plant J., 2006. 48(5): p. 647-56
  16. Kesarwani M,Yoo J,Dong X
    Genetic interactions of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis.
    Plant Physiol., 2007. 144(1): p. 336-46
  17. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  18. Li S, et al.
    Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana.
    Plant Cell, 2009. 21(2): p. 429-41
  19. Farinati S,DalCorso G,Varotto S,Furini A
    The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants.
    New Phytol., 2010. 185(4): p. 964-78
  20. Choi J, et al.
    The cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis.
    Dev. Cell, 2010. 19(2): p. 284-95
  21. Li S,Gutsche N,Zachgo S
    The ROXY1 C-terminal L**LL motif is essential for the interaction with TGA transcription factors.
    Plant Physiol., 2011. 157(4): p. 2056-68
  22. Windram O, et al.
    Arabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis.
    Plant Cell, 2012. 24(9): p. 3530-57
  23. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  24. Gangadharan A,Sreerekha MV,Whitehill J,Ham JH,Mackey D
    The Pseudomonas syringae pv. tomato type III effector HopM1 suppresses Arabidopsis defenses independent of suppressing salicylic acid signaling and of targeting AtMIN7.
    PLoS ONE, 2013. 8(12): p. e82032
  25. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  26. Herrera-Vásquez A, et al.
    Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis.
    Plant Mol. Biol. Rep., 2018.
  27. Fang H, et al.
    The Ca2+ /calmodulin2-binding transcription factor TGA3 elevates LCD expression and H2 S production to bolster Cr6+ tolerance in Arabidopsis.
    Plant J., 2017. 91(6): p. 1038-1050
  28. Sarkar S, et al.
    Interaction of Arabidopsis TGA3 and WRKY53 transcription factors on Cestrum yellow leaf curling virus (CmYLCV) promoter mediates salicylic acid-dependent gene expression in planta.
    Planta, 2018. 247(1): p. 181-199
  29. Lam E,Lam YK
    Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity.
    Nucleic Acids Res., 1995. 23(18): p. 3778-85
  30. Miao ZH,Lam E
    Targeted disruption of the TGA3 locus in Arabidopsis thaliana.
    Plant J., 1995. 7(2): p. 359-65
  31. Miao ZH,Liu X,Lam E
    TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.
    Plant Mol. Biol., 1994. 25(1): p. 1-11
  32. de Pater S,Pham K,Memelink J,Kijne J
    Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2.
    Mol. Gen. Genet., 1996. 250(2): p. 237-9
  33. Szymanski DB,Liao B,Zielinski RE
    Calmodulin isoforms differentially enhance the binding of cauliflower nuclear proteins and recombinant TGA3 to a region derived from the Arabidopsis Cam-3 promoter.
    Plant Cell, 1996. 8(6): p. 1069-77