PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G06160.1
Common NameERF094, F9P14.2, ORA59
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 244aa    MW: 27089.8 Da    PI: 4.8288
Description octadecanoid-responsive Arabidopsis AP2/ERF 59
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G06160.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP255.81.2e-1781131255
          AP2   2 gykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                   y+GVr ++ +g+++AeIrd    + k  r++lg+f+tae Aa a+++a+ +l+g
  AT1G06160.1  81 SYRGVRKRP-WGKFAAEIRDsT--R-KGIRVWLGTFDTAEAAALAYDQAAFALKG 131
                  69*******.**********32..2.24***********************9987 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000186.41E-2180141No hitNo description
SuperFamilySSF541717.19E-2281141IPR016177DNA-binding domain
Gene3DG3DSA:3.30.730.106.6E-3081139IPR001471AP2/ERF domain
PfamPF008471.2E-1281131IPR001471AP2/ERF domain
SMARTSM003801.0E-3781145IPR001471AP2/ERF domain
PROSITE profilePS5103223.14381139IPR001471AP2/ERF domain
PRINTSPR003671.4E-108293IPR001471AP2/ERF domain
PRINTSPR003671.4E-10105121IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009723Biological Processresponse to ethylene
GO:0009753Biological Processresponse to jasmonic acid
GO:0009861Biological Processjasmonic acid and ethylene-dependent systemic resistance
GO:0009873Biological Processethylene-activated signaling pathway
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009025anatomyvascular leaf
PO:0009047anatomystem
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
Sequence ? help Back to Top
Protein Sequence    Length: 244 aa     Download sequence    Send to blast
MEYQTNFLSG EFSPENSSSS SWSSQESFLW EESFLHQSFD QSFLLSSPTD NYCDDFFAFE  60
SSIIKEEGKE ATVAAEEEEK SYRGVRKRPW GKFAAEIRDS TRKGIRVWLG TFDTAEAAAL  120
AYDQAAFALK GSLAVLNFPA DVVEESLRKM ENVNLNDGES PVIALKRKHS MRNRPRGKKK  180
SSSSSTLTSS PSSSSSYSSS SSSSSLSSRS RKQSVVMTQE SNTTLVVLED LGAEYLEELM  240
RSCS
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5wx9_A9e-27781391172Ethylene-responsive transcription factor ERF096
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.423540.0leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible260783_at0.0
Expression AtlasAT1G06160-
AtGenExpressAT1G06160-
ATTED-IIAT1G06160-
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-3 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 18 members in this subfamily including ATERF-1, ATERF-2, AND ATERF-5.
UniProtProbably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Acts as an essential integrator of the JA and ethylene signal transduction pathways. Activates the expression of the PDF1.2A gene. {ECO:0000269|PubMed:18467450}.
Function -- GeneRIF ? help Back to Top
  1. ORA59 (AT1g06160) is an essential integrator of the jasmonic acid and ethylene signal transduction pathways.
    [PMID: 18467450]
  2. ORA59 and two functionally equivalent GCC box binding sites form the module that enables the PDF1.2 gene to respond synergistically to simultaneous activation of the jasmonate and ethylene signaling pathways
    [PMID: 21246258]
  3. Salicylic acid exerts a negative effect on the accumulation of the transcription factor ORA59.
    [PMID: 23435661]
  4. the opposing function of two transcription factors, ethylene insensitive3 (EIN3) and EIN3-Like1 (EIL1), in salicylic acid-mediated suppression and jasmonic acid-mediated activation of PLANT DEFENSIN1.2 (PDF1.2), through ORA59.
    [PMID: 28168848]
  5. enables agmatine coumaryl transferase gene expression and hydroxycinnamic acid amides biosynthesis to respond to simultaneous activation of the JA/ET signaling pathways
    [PMID: 29752755]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00023PBM25215497Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G06160.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT2G38470 (R), AT5G47220 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT3G12500(A), AT5G44420(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDethylene
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G06160
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankBT0110010.0BT011001.1 Arabidopsis thaliana At1g06160 gene, complete cds.
GenBankBT0149580.0BT014958.1 Arabidopsis thaliana At1g06160 gene, complete cds.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
GenBankF9P140.0AC025290.3 Sequence of BAC F9P14 from Arabidopsis thaliana chromosome 1, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_172106.11e-176octadecanoid-responsive AP2/ERF 59
SwissprotQ9LND11e-177ERF94_ARATH; Ethylene-responsive transcription factor ERF094
TrEMBLA0A178WAR01e-175A0A178WAR0_ARATH; ORA59
STRINGAT1G06160.11e-176(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM39901859
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Fujimoto SY,Ohta M,Usui A,Shinshi H,Ohme-Takagi M
    Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression.
    Plant Cell, 2000. 12(3): p. 393-404
    [PMID:10715325]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. O
    Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection.
    Plant Physiol., 2002. 128(4): p. 1313-22
    [PMID:11950980]
  4. McGrath KC, et al.
    Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression.
    Plant Physiol., 2005. 139(2): p. 949-59
    [PMID:16183832]
  5. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  6. Thilmony R,Underwood W,He SY
    Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.
    Plant J., 2006. 46(1): p. 34-53
    [PMID:16553894]
  7. Nemhauser JL,Hong F,Chory J
    Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses.
    Cell, 2006. 126(3): p. 467-75
    [PMID:16901781]
  8. Hectors K,Prinsen E,De Coen W,Jansen MA,Guisez Y
    Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation show specific changes in morphology and gene expression in the absence of stress symptoms.
    New Phytol., 2007. 175(2): p. 255-70
    [PMID:17587374]
  9. Dombrecht B, et al.
    MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis.
    Plant Cell, 2007. 19(7): p. 2225-45
    [PMID:17616737]
  10. Veyres N, et al.
    The Arabidopsis sweetie mutant is affected in carbohydrate metabolism and defective in the control of growth, development and senescence.
    Plant J., 2008. 55(4): p. 665-86
    [PMID:18452589]
  11. Pré M, et al.
    The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense.
    Plant Physiol., 2008. 147(3): p. 1347-57
    [PMID:18467450]
  12. Memelink J
    Regulation of gene expression by jasmonate hormones.
    Phytochemistry, 2009. 70(13-14): p. 1560-70
    [PMID:19796781]
  13. Zander M,La Camera S,Lamotte O,Métraux JP,Gatz C
    Arabidopsis thaliana class-II TGA transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses.
    Plant J., 2010. 61(2): p. 200-10
    [PMID:19832945]
  14. Leon-Reyes A, et al.
    Ethylene signaling renders the jasmonate response of Arabidopsis insensitive to future suppression by salicylic Acid.
    Mol. Plant Microbe Interact., 2010. 23(2): p. 187-97
    [PMID:20064062]
  15. Zarei A, et al.
    Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF1.2 promoter in Arabidopsis.
    Plant Mol. Biol., 2011. 75(4-5): p. 321-31
    [PMID:21246258]
  16. Zhang W, et al.
    LeERF-1, a novel AP2/ERF family gene within the B3 subcluster, is down-regulated by light signals in Lithospermum erythrorhizon.
    Plant Biol (Stuttg), 2011. 13(2): p. 343-8
    [PMID:21309981]
  17. Zander M,Chen S,Imkampe J,Thurow C,Gatz C
    Repression of the Arabidopsis thaliana jasmonic acid/ethylene-induced defense pathway by TGA-interacting glutaredoxins depends on their C-terminal ALWL motif.
    Mol Plant, 2012. 5(4): p. 831-40
    [PMID:22207719]
  18. Verhage A, et al.
    Rewiring of the Jasmonate Signaling Pathway in Arabidopsis during Insect Herbivory.
    Front Plant Sci, 2011. 2: p. 47
    [PMID:22645537]
  19. Lu X, et al.
    Characterization of a novel ERF transcription factor in Artemisia annua and its induction kinetics after hormones and stress treatments.
    Mol. Biol. Rep., 2012. 39(10): p. 9521-7
    [PMID:22714923]

  20. MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis.
    Plant Physiol., 2012. 160(1): p. 541-55
    [PMID:22822211]
  21. Van der Does D, et al.
    Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ by targeting GCC promoter motifs via transcription factor ORA59.
    Plant Cell, 2013. 25(2): p. 744-61
    [PMID:23435661]
  22. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  23. Scala A, et al.
    E-2-hexenal promotes susceptibility to Pseudomonas syringae by activating jasmonic acid pathways in Arabidopsis.
    Front Plant Sci, 2013. 4: p. 74
    [PMID:23630530]
  24. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  25. Zander M,Thurow C,Gatz C
    TGA Transcription Factors Activate the Salicylic Acid-Suppressible Branch of the Ethylene-Induced Defense Program by Regulating ORA59 Expression.
    Plant Physiol., 2014. 165(4): p. 1671-1683
    [PMID:24989234]
  26. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  27. Wang F, et al.
    TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana.
    Front Plant Sci, 2015. 6: p. 108
    [PMID:25774162]
  28. Lehmeyer M, et al.
    Functional dissection of a strong and specific microbe-associated molecular pattern-responsive synthetic promoter.
    Plant Biotechnol. J., 2016. 14(1): p. 61-71
    [PMID:25819608]
  29. Caarls L,Pieterse CM,Van Wees SC
    How salicylic acid takes transcriptional control over jasmonic acid signaling.
    Front Plant Sci, 2015. 6: p. 170
    [PMID:25859250]
  30. Wang C, et al.
    The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum.
    Plant Physiol., 2015. 169(1): p. 856-72
    [PMID:26143252]
  31. Wang C, et al.
    Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola.
    Plant J., 2015. 83(6): p. 1019-33
    [PMID:26216741]
  32. Schmiesing A,Emonet A,Gouhier-Darimont C,Reymond P
    Arabidopsis MYC Transcription Factors Are the Target of Hormonal Salicylic Acid/Jasmonic Acid Cross Talk in Response to Pieris brassicae Egg Extract.
    Plant Physiol., 2016. 170(4): p. 2432-43
    [PMID:26884488]
  33. Caarls L, et al.
    Assessing the Role of ETHYLENE RESPONSE FACTOR Transcriptional Repressors in Salicylic Acid-Mediated Suppression of Jasmonic Acid-Responsive Genes.
    Plant Cell Physiol., 2017. 58(2): p. 266-278
    [PMID:27837094]
  34. He X,Jiang J,Wang CQ,Dehesh K
    ORA59 and EIN3 interaction couples jasmonate-ethylene synergistic action to antagonistic salicylic acid regulation of PDF expression.
    J Integr Plant Biol, 2017. 59(4): p. 275-287
    [PMID:28168848]
  35. Van den Broeck L, et al.
    From network to phenotype: the dynamic wiring of an Arabidopsis transcriptional network induced by osmotic stress.
    Mol. Syst. Biol., 2017. 13(12): p. 961
    [PMID:29269383]
  36. Li X,Yang R,Chen H
    The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA.
    PLoS ONE, 2018. 13(3): p. e0193458
    [PMID:29513733]
  37. Li J, et al.
    Jasmonic acid/ethylene signaling coordinates hydroxycinnamic acid amides biosynthesis through ORA59 transcription factor.
    Plant J., 2018. 95(3): p. 444-457
    [PMID:29752755]
  38. Riechmann JL,Meyerowitz EM
    The AP2/EREBP family of plant transcription factors.
    Biol. Chem., 1998. 379(6): p. 633-46
    [PMID:9687012]