PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G02065.2
Common NameSPL8
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family SBP
Protein Properties Length: 246aa    MW: 27504.1 Da    PI: 7.3421
Description squamosa promoter binding protein-like 8
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G02065.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          SBP   1 lCqvegCeadlseakeyhrrhkvCevhskapvvlvsgleqrfCqqCsrfh 50 
                  6************************************************5 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:4.10.1100.101.1E-26182236IPR004333Transcription factor, SBP-box
PROSITE profilePS5114119.854185246IPR004333Transcription factor, SBP-box
SuperFamilySSF1036121.09E-23186236IPR004333Transcription factor, SBP-box
PfamPF031104.7E-19188237IPR004333Transcription factor, SBP-box
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009554Biological Processmegasporogenesis
GO:0009556Biological Processmicrosporogenesis
GO:0030154Biological Processcell differentiation
GO:0048653Biological Processanther development
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0046872Molecular Functionmetal ion binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
Sequence ? help Back to Top
Protein Sequence    Length: 246 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wj0_A4e-19188235653squamosa promoter-binding protein-like 12
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.4990.0floral meristem| flower
Expression -- Microarray ? help Back to Top
Source ID
Expression AtlasAT1G02065
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed during plant development. During pollen development, required for crosporogenesis that leads to archesporial formation and for the histogenesis of the microsporangia. During ovules development, involved in the transition to meiosis of the megaspore mother cells. {ECO:0000269|PubMed:10524240, ECO:0000269|PubMed:12671094}.
UniprotTISSUE SPECIFICITY: Expressed in shoot apical region and early floral tissues. Transcripts levels increase in developing pollen sacs, and decrease in later stage of anther development. Strongly expressed in the placental region of the carpels. {ECO:0000269|PubMed:12671094, ECO:0000269|PubMed:17093870}.
Functional Description ? help Back to Top
Source Description
TAIREncodes an SBP-box gene, a member of the SPL gene family. Mutants are affected in micro- and megasporogenesis, trichome formation on sepals, and stamen filament elongation.
UniProtTrans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3'. Binds specifically to the 5'-GTAC-3' core sequence. Involved in development and floral organogenesis. Required for ovule differentiation, pollen production, filament elongation, seed formation and siliques elongation. Also seems to play a role in the formation of trichomes on sepals. May positively modulate gibberellin (GA) signaling in flower. {ECO:0000269|PubMed:12671094, ECO:0000269|PubMed:16095614, ECO:0000269|PubMed:17093870}.
Function -- GeneRIF ? help Back to Top
  1. Data demonstrate that miR156 is a quantitative, rather than spatial, modulator of SPL8 expression in leaf primordia and that SPL6 activity nonautonomously inhibits initiation of new leaves at the shoot apical meristem.
    [PMID: 18492871]
  2. SPL8 and miR156-targeted SPL genes control gynoecium patterning through interference with auxin homeostasis and signalling.
    [PMID: 23621152]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Interaction ? help Back to Top
Source Intact With
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G02065
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ0116410.0AJ011641.1 Arabidopsis thaliana (ecotype Columbia) spl8 gene, exons 1-3.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
GenBankU899590.0U89959.1 Arabidopsis thaliana BAC T7I23, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_973738.10.0squamosa promoter binding protein-like 8
SwissprotQ8GXL31e-176SPL8_ARATH; Squamosa promoter-binding-like protein 8
TrEMBLQ3EDL20.0Q3EDL2_ARATH; Squamosa promoter binding protein-like 8
STRINGAT1G02065.11e-174(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Unte US, et al.
    SPL8, an SBP-box gene that affects pollen sac development in Arabidopsis.
    Plant Cell, 2003. 15(4): p. 1009-19
  2. Folta KM,Pontin MA,Karlin-Neumann G,Bottini R,Spalding EP
    Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light.
    Plant J., 2003. 36(2): p. 203-14
  3. Albert VA, et al.
    Floral gene resources from basal angiosperms for comparative genomics research.
    BMC Plant Biol., 2005. 5: p. 5
  4. Birkenbihl RP,Jach G,Saedler H,Huijser P
    Functional dissection of the plant-specific SBP-domain: overlap of the DNA-binding and nuclear localization domains.
    J. Mol. Biol., 2005. 352(3): p. 585-96
  5. Zhang Y,Schwarz S,Saedler H,Huijser P
    SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis.
    Plant Mol. Biol., 2007. 63(3): p. 429-39
  6. Xing S,Zachgo S
    ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development.
    Plant J., 2008. 53(5): p. 790-801
  7. Wang JW,Schwab R,Czech B,Mica E,Weigel D
    Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana.
    Plant Cell, 2008. 20(5): p. 1231-43
  8. Xing S,Salinas M,H
    miR156-targeted and nontargeted SBP-box transcription factors act in concert to secure male fertility in Arabidopsis.
    Plant Cell, 2010. 22(12): p. 3935-50
  9. Smaczniak C, et al.
    Characterization of MADS-domain transcription factor complexes in Arabidopsis flower development.
    Proc. Natl. Acad. Sci. U.S.A., 2012. 109(5): p. 1560-5
  10. Xing S, et al.
    SPL8 and miR156-targeted SPL genes redundantly regulate Arabidopsis gynoecium differential patterning.
    Plant J., 2013. 75(4): p. 566-77
  11. Jorgensen SA,Preston JC
    Differential SPL gene expression patterns reveal candidate genes underlying flowering time and architectural differences in Mimulus and Arabidopsis.
    Mol. Phylogenet. Evol., 2014. 73: p. 129-39
  12. Debernardi JM, et al.
    Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity.
    Plant J., 2014. 79(3): p. 413-26
  13. Xing S, et al.
    SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility.
    Plants (Basel), 2013. 2(3): p. 416-28