PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Vigna angularis
HSF Family
Species TF ID Description
Vang0134s00610.1HSF family protein
Vang01g04300.1HSF family protein
Vang01g17680.1HSF family protein
Vang0236s00410.1HSF family protein
Vang02g04080.1HSF family protein
Vang02g08480.1HSF family protein
Vang05g04200.1HSF family protein
Vang06g04820.1HSF family protein
Vang06g05270.1HSF family protein
Vang06g17230.1HSF family protein
Vang06g19780.1HSF family protein
Vang06g22230.1HSF family protein
Vang06g25790.1HSF family protein
Vang07g02020.1HSF family protein
Vang07g05460.1HSF family protein
Vang10g00980.1HSF family protein
Vang1163s00010.1HSF family protein
Vang11g02160.1HSF family protein
Vang11g03820.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645