PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Triticum urartu
HSF Family
Species TF ID Description
TRIUR3_02308-P1HSF family protein
TRIUR3_08676-P1HSF family protein
TRIUR3_10874-P1HSF family protein
TRIUR3_11025-P1HSF family protein
TRIUR3_12841-P1HSF family protein
TRIUR3_18146-P1HSF family protein
TRIUR3_19198-P1HSF family protein
TRIUR3_19594-P1HSF family protein
TRIUR3_24476-P1HSF family protein
TRIUR3_25943-P1HSF family protein
TRIUR3_26103-P1HSF family protein
TRIUR3_27052-P1HSF family protein
TRIUR3_30232-P1HSF family protein
TRIUR3_31165-P1HSF family protein
TRIUR3_33705-P1HSF family protein
TRIUR3_33712-P1HSF family protein
TRIUR3_34250-P1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645