PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Tarenaya hassleriana
FAR1 Family
Species TF ID Description
XP_010520853.1FAR1 family protein
XP_010521607.1FAR1 family protein
XP_010522310.1FAR1 family protein
XP_010522311.1FAR1 family protein
XP_010522312.1FAR1 family protein
XP_010522313.1FAR1 family protein
XP_010524153.1FAR1 family protein
XP_010524725.1FAR1 family protein
XP_010524779.1FAR1 family protein
XP_010524780.1FAR1 family protein
XP_010524782.1FAR1 family protein
XP_010526071.1FAR1 family protein
XP_010527022.1FAR1 family protein
XP_010527352.1FAR1 family protein
XP_010532500.1FAR1 family protein
XP_010532501.1FAR1 family protein
XP_010532502.1FAR1 family protein
XP_010532503.1FAR1 family protein
XP_010532504.1FAR1 family protein
XP_010536055.1FAR1 family protein
XP_010536056.1FAR1 family protein
XP_010536794.1FAR1 family protein
XP_010536802.1FAR1 family protein
XP_010536810.1FAR1 family protein
XP_010536819.1FAR1 family protein
XP_010536829.1FAR1 family protein
XP_010536837.1FAR1 family protein
XP_010536844.1FAR1 family protein
XP_010543997.1FAR1 family protein
XP_010543998.1FAR1 family protein
XP_010543999.1FAR1 family protein
XP_010544000.1FAR1 family protein
XP_010545749.1FAR1 family protein
XP_010546892.1FAR1 family protein
XP_010546893.1FAR1 family protein
XP_010546894.1FAR1 family protein
XP_010547203.1FAR1 family protein
XP_010551740.1FAR1 family protein
XP_010551787.1FAR1 family protein
XP_010551788.1FAR1 family protein
XP_010551789.1FAR1 family protein
XP_010551790.1FAR1 family protein
XP_010551791.1FAR1 family protein
XP_010551792.1FAR1 family protein
XP_010551793.1FAR1 family protein
XP_010551794.1FAR1 family protein
XP_010551795.1FAR1 family protein
XP_010551796.1FAR1 family protein
XP_010551798.1FAR1 family protein
XP_010553350.1FAR1 family protein
XP_010555162.1FAR1 family protein
XP_010557748.1FAR1 family protein
XP_010557750.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885