PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Solanum pimpinellifolium
HSF Family
Species TF ID Description
Sopim02g072000.0.1HSF family protein
Sopim02g072060.0.1HSF family protein
Sopim02g078340.0.1HSF family protein
Sopim02g079180.0.1HSF family protein
Sopim02g090820.0.1HSF family protein
Sopim03g006000.0.1HSF family protein
Sopim03g026020.0.1HSF family protein
Sopim03g097120.0.1HSF family protein
Sopim04g016000.0.1HSF family protein
Sopim04g078770.0.1HSF family protein
Sopim06g053960.0.1HSF family protein
Sopim06g072750.0.1HSF family protein
Sopim07g040680.0.1HSF family protein
Sopim07g055710.0.1HSF family protein
Sopim08g005170.0.1HSF family protein
Sopim08g062960.0.1HSF family protein
Sopim08g076590.0.1HSF family protein
Sopim08g080540.0.1HSF family protein
Sopim09g009100.0.1HSF family protein
Sopim09g059520.0.1HSF family protein
Sopim09g065660.0.1HSF family protein
Sopim10g079380.0.1HSF family protein
Sopim11g064990.0.1HSF family protein
Sopim12g007070.0.1HSF family protein
Sopim12g098520.0.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645