PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Sesamum indicum
HSF Family
Species TF ID Description
XP_011070307.1HSF family protein
XP_011070552.1HSF family protein
XP_011071260.1HSF family protein
XP_011073463.1HSF family protein
XP_011073785.1HSF family protein
XP_011073942.1HSF family protein
XP_011074417.1HSF family protein
XP_011075334.1HSF family protein
XP_011075335.1HSF family protein
XP_011076201.1HSF family protein
XP_011076202.1HSF family protein
XP_011078395.1HSF family protein
XP_011078910.1HSF family protein
XP_011079918.1HSF family protein
XP_011081009.1HSF family protein
XP_011082584.1HSF family protein
XP_011082962.1HSF family protein
XP_011083880.1HSF family protein
XP_011084402.1HSF family protein
XP_011084620.1HSF family protein
XP_011091032.1HSF family protein
XP_011094419.1HSF family protein
XP_011096226.1HSF family protein
XP_011096299.1HSF family protein
XP_011098060.1HSF family protein
XP_011099060.1HSF family protein
XP_011099137.1HSF family protein
XP_011101442.1HSF family protein
XP_011101443.1HSF family protein
XP_011101446.1HSF family protein
XP_011101447.1HSF family protein
XP_011101504.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645