PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Raphanus sativus
FAR1 Family
Species TF ID Description
Rsa1.0_00049.1_g00016.1FAR1 family protein
Rsa1.0_00510.1_g00015.1FAR1 family protein
Rsa1.0_00512.1_g00002.1FAR1 family protein
Rsa1.0_00515.1_g00016.1FAR1 family protein
Rsa1.0_00605.1_g00016.1FAR1 family protein
Rsa1.0_00685.1_g00005.1FAR1 family protein
Rsa1.0_00915.1_g00009.1FAR1 family protein
Rsa1.0_00962.1_g00008.1FAR1 family protein
Rsa1.0_01070.1_g00010.1FAR1 family protein
Rsa1.0_01456.1_g00005.1FAR1 family protein
Rsa1.0_02020.1_g00004.1FAR1 family protein
Rsa1.0_02096.1_g00005.1FAR1 family protein
Rsa1.0_03345.1_g00005.1FAR1 family protein
Rsa1.0_03577.1_g00002.1FAR1 family protein
Rsa1.0_04711.1_g00001.1FAR1 family protein
Rsa1.0_05746.1_g00002.1FAR1 family protein
Rsa1.0_07458.1_g00001.1FAR1 family protein
Rsa1.0_07901.1_g00001.1FAR1 family protein
Rsa1.0_14432.1_g00002.1FAR1 family protein
Rsa1.0_15698.1_g00001.1FAR1 family protein
Rsa1.0_26083.1_g00001.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885