PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Raphanus raphanistrum
HSF Family
Species TF ID Description
RrC10193_p1HSF family protein
RrC10492_p2HSF family protein
RrC11089_p3HSF family protein
RrC11096_p1HSF family protein
RrC11149_p1HSF family protein
RrC11228_p1HSF family protein
RrC11824_p1HSF family protein
RrC126_p8HSF family protein
RrC13169_p1HSF family protein
RrC13210_p2HSF family protein
RrC1432_p5HSF family protein
RrC14701_p1HSF family protein
RrC14908_p1HSF family protein
RrC1575_p2HSF family protein
RrC16149_p1HSF family protein
RrC1799_p1HSF family protein
RrC18473_p1HSF family protein
RrC1881_p4HSF family protein
RrC190_p6HSF family protein
RrC190_p7HSF family protein
RrC2058_p3HSF family protein
RrC22249_p2HSF family protein
RrC2480_p5HSF family protein
RrC2679_p2HSF family protein
RrC297_p3HSF family protein
RrC3106_p1HSF family protein
RrC3289_p1HSF family protein
RrC3419_p2HSF family protein
RrC3959_p4HSF family protein
RrC4174_p1HSF family protein
RrC43086_p1HSF family protein
RrC4475_p1HSF family protein
RrC4790_p2HSF family protein
RrC4790_p4HSF family protein
RrC5221_p2HSF family protein
RrC5581_p1HSF family protein
RrC5698_p2HSF family protein
RrC6094_p3HSF family protein
RrC6452_p4HSF family protein
RrC6561_p1HSF family protein
RrC6582_p2HSF family protein
RrC7060_p2HSF family protein
RrC877_p3HSF family protein
RrC877_p5HSF family protein
RrC883_p2HSF family protein
RrC9878_p1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645