PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Phyllostachys heterocycla
HSF Family
Species TF ID Description
PH01000000G3800HSF family protein
PH01000018G0140HSF family protein
PH01000081G0140HSF family protein
PH01000149G1320HSF family protein
PH01000174G0590HSF family protein
PH01000194G0800HSF family protein
PH01000208G0690HSF family protein
PH01000294G0540HSF family protein
PH01000314G0470HSF family protein
PH01000333G0160HSF family protein
PH01000371G0730HSF family protein
PH01000436G0570HSF family protein
PH01000456G0310HSF family protein
PH01000546G0840HSF family protein
PH01000626G0430HSF family protein
PH01000700G0690HSF family protein
PH01000701G0030HSF family protein
PH01000746G0370HSF family protein
PH01000814G0580HSF family protein
PH01000849G0330HSF family protein
PH01001018G0530HSF family protein
PH01001028G0180HSF family protein
PH01001228G0360HSF family protein
PH01001554G0080HSF family protein
PH01001554G0310HSF family protein
PH01001858G0120HSF family protein
PH01002437G0340HSF family protein
PH01002606G0040HSF family protein
PH01003169G0070HSF family protein
PH01003916G0010HSF family protein
PH01004959G0060HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645