PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Oryza punctata
HSF Family
Species TF ID Description
OPUNC01G15930.1HSF family protein
OPUNC01G20490.1HSF family protein
OPUNC01G23470.1HSF family protein
OPUNC01G27840.1HSF family protein
OPUNC01G29770.1HSF family protein
OPUNC02G08730.1HSF family protein
OPUNC02G15430.1HSF family protein
OPUNC02G16840.1HSF family protein
OPUNC03G04310.1HSF family protein
OPUNC03G08660.1HSF family protein
OPUNC03G08660.2HSF family protein
OPUNC03G17650.1HSF family protein
OPUNC03G21100.1HSF family protein
OPUNC03G31000.1HSF family protein
OPUNC03G34370.1HSF family protein
OPUNC03G38490.1HSF family protein
OPUNC04G19790.1HSF family protein
OPUNC05G21050.1HSF family protein
OPUNC06G16360.1HSF family protein
OPUNC06G17020.1HSF family protein
OPUNC07G04790.1HSF family protein
OPUNC07G22520.1HSF family protein
OPUNC08G15560.1HSF family protein
OPUNC08G20670.1HSF family protein
OPUNC09G11010.1HSF family protein
OPUNC09G11110.1HSF family protein
OPUNC09G15460.1HSF family protein
OPUNC10G08860.1HSF family protein
OPUNC10G08860.2HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645