PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Oryza glumaepatula
HSF Family
Species TF ID Description
OGLUM01G23720.1HSF family protein
OGLUM01G27120.1HSF family protein
OGLUM01G35030.1HSF family protein
OGLUM02G09850.1HSF family protein
OGLUM02G17550.1HSF family protein
OGLUM02G19090.1HSF family protein
OGLUM02G19090.2HSF family protein
OGLUM02G19090.3HSF family protein
OGLUM02G19090.4HSF family protein
OGLUM03G04480.1HSF family protein
OGLUM03G09110.1HSF family protein
OGLUM03G19040.1HSF family protein
OGLUM03G33530.1HSF family protein
OGLUM03G33530.2HSF family protein
OGLUM03G33530.3HSF family protein
OGLUM03G37030.1HSF family protein
OGLUM03G41290.1HSF family protein
OGLUM04G22060.1HSF family protein
OGLUM05G25010.1HSF family protein
OGLUM06G19290.1HSF family protein
OGLUM06G19920.1HSF family protein
OGLUM07G04480.1HSF family protein
OGLUM07G23940.1HSF family protein
OGLUM08G18590.1HSF family protein
OGLUM08G23900.1HSF family protein
OGLUM09G13090.1HSF family protein
OGLUM09G13200.1HSF family protein
OGLUM09G17660.1HSF family protein
OGLUM09G17660.2HSF family protein
OGLUM10G10060.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645