PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Oryza brachyantha
FAR1 Family
Species TF ID Description
OB0054G10020.1FAR1 family protein
OB0054G10030.1FAR1 family protein
OB01G18260.1FAR1 family protein
OB01G25460.1FAR1 family protein
OB02G17140.1FAR1 family protein
OB02G20370.1FAR1 family protein
OB02G21030.1FAR1 family protein
OB02G21340.1FAR1 family protein
OB02G27370.1FAR1 family protein
OB02G27690.1FAR1 family protein
OB02G44380.1FAR1 family protein
OB03G16050.1FAR1 family protein
OB03G21170.1FAR1 family protein
OB03G21220.1FAR1 family protein
OB03G34250.1FAR1 family protein
OB03G35710.1FAR1 family protein
OB03G39100.1FAR1 family protein
OB03G43000.1FAR1 family protein
OB03G47710.1FAR1 family protein
OB04G19620.1FAR1 family protein
OB04G20960.1FAR1 family protein
OB04G23370.1FAR1 family protein
OB04G32220.1FAR1 family protein
OB06G14070.1FAR1 family protein
OB06G27780.1FAR1 family protein
OB06G34800.1FAR1 family protein
OB07G20960.1FAR1 family protein
OB07G21030.1FAR1 family protein
OB07G24510.1FAR1 family protein
OB07G27730.1FAR1 family protein
OB08G14200.1FAR1 family protein
OB09G10870.1FAR1 family protein
OB10G13790.1FAR1 family protein
OB12G10090.1FAR1 family protein
OB12G13670.1FAR1 family protein
OB12G16010.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885