PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Nicotiana tabacum
SBP Family
Species TF ID Description
XP_016433751.1SBP family protein
XP_016436160.1SBP family protein
XP_016436950.1SBP family protein
XP_016437596.1SBP family protein
XP_016437677.1SBP family protein
XP_016437679.1SBP family protein
XP_016439329.1SBP family protein
XP_016441061.1SBP family protein
XP_016441586.1SBP family protein
XP_016443563.1SBP family protein
XP_016443564.1SBP family protein
XP_016443565.1SBP family protein
XP_016443566.1SBP family protein
XP_016443567.1SBP family protein
XP_016443568.1SBP family protein
XP_016445745.1SBP family protein
XP_016449891.1SBP family protein
XP_016449892.1SBP family protein
XP_016453703.1SBP family protein
XP_016458284.1SBP family protein
XP_016458285.1SBP family protein
XP_016458286.1SBP family protein
XP_016458287.1SBP family protein
XP_016458288.1SBP family protein
XP_016458289.1SBP family protein
XP_016458291.1SBP family protein
XP_016458292.1SBP family protein
XP_016458293.1SBP family protein
XP_016458294.1SBP family protein
XP_016458295.1SBP family protein
XP_016458296.1SBP family protein
XP_016458950.1SBP family protein
XP_016461913.1SBP family protein
XP_016461914.1SBP family protein
XP_016462352.1SBP family protein
XP_016466876.1SBP family protein
XP_016466919.1SBP family protein
XP_016467173.1SBP family protein
XP_016474508.1SBP family protein
XP_016474509.1SBP family protein
XP_016476532.1SBP family protein
XP_016476533.1SBP family protein
XP_016481556.1SBP family protein
XP_016481940.1SBP family protein
XP_016482449.1SBP family protein
XP_016482450.1SBP family protein
XP_016482451.1SBP family protein
XP_016482452.1SBP family protein
XP_016482453.1SBP family protein
XP_016485233.1SBP family protein
XP_016485234.1SBP family protein
XP_016485336.1SBP family protein
XP_016486218.1SBP family protein
XP_016486384.1SBP family protein
XP_016488455.1SBP family protein
XP_016489922.1SBP family protein
XP_016491740.1SBP family protein
XP_016495494.1SBP family protein
XP_016498370.1SBP family protein
XP_016498564.1SBP family protein
XP_016498570.1SBP family protein
XP_016498962.1SBP family protein
XP_016502562.1SBP family protein
XP_016504684.1SBP family protein
XP_016504693.1SBP family protein
XP_016504699.1SBP family protein
XP_016504706.1SBP family protein
XP_016504711.1SBP family protein
XP_016504715.1SBP family protein
XP_016504721.1SBP family protein
XP_016505306.1SBP family protein
XP_016506660.1SBP family protein
XP_016506661.1SBP family protein
XP_016506662.1SBP family protein
XP_016506663.1SBP family protein
XP_016507693.1SBP family protein
XP_016509383.1SBP family protein
XP_016510730.1SBP family protein
XP_016510731.1SBP family protein
XP_016512846.1SBP family protein
XP_016514544.1SBP family protein
XP_016515187.1SBP family protein
SBP Family Introduction

SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Ishizuka Y, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors
J Mol Biol. 2004 Mar 12;337(1):49-63.
PMID: 15001351

SBP-box genes were first characterized as SQUAMOSA promoter binding proteins (SBPs) to regulate the expression of MADS-box genes in early flower development of A. majus (Klein et al., 1996). Since then, SBP-box genes have been identified in many plants including green alga, moss, silver birch, A. majus, Arabidopsis and maize. They play critical roles in regulating flower and fruit development as well as other physiological processes ([Moreno et al., 1997],[Eriksson et al., 2004], [Lannenpaa et al., 2004], [Arazi et al., 2005] and [Kropat et al., 2005]). It has been reported that Arabidopsis SPL3, SPL8 and SPL14 involves in flowering, sporogenesis, GA signaling and toxin resistance ([Cardon et al., 1997], [Unte et al., 2003], [Stone et al., 2005] and [Zhang et al., 2006]) while maize tga1 and tomato LeSPL-CNR affect fruit development ([Wang et al., 2005a] and [Manning et al., 2006]).

SBP-box genes encode proteins sharing a conserved DNA-binding domain of 79 amino acid residues. It has been proved that the DNA-binding domain of SBP-box genes is necessary and sufficient to bind to a palindromic GTAC core motif ([Klein et al., 1996], [Cardon et al., 1997], [Cardon et al., 1999], [Lannenpaa et al., 2004] and [Birkenbihl et al., 2005]). Studies on the NMR solution structure of the fragment of Arabidopsis SPL4 and SPL7 revealed that the DNA-binding domain of SBPs consisted of two separate zinc-binding sites. One zinc finger is C3H or C4 and the other is C2HC (Yamasaki et al., 2004 K. Yamasaki et al.,).

Guo AY, Zhu QH, Gu X, Ge S, Yang J, Luo J.
Genome-wide identification and evolutionary analysis of the plant specific SBP-box transcription factor family.
Gene. 2008 Jul 15;418(1-2):1-8.
PMID: 18495384