PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Nicotiana tabacum
HSF Family
Species TF ID Description
XP_016433783.1HSF family protein
XP_016434163.1HSF family protein
XP_016434894.1HSF family protein
XP_016439999.1HSF family protein
XP_016440966.1HSF family protein
XP_016443432.1HSF family protein
XP_016443433.1HSF family protein
XP_016444574.1HSF family protein
XP_016448302.1HSF family protein
XP_016448505.1HSF family protein
XP_016448515.1HSF family protein
XP_016448516.1HSF family protein
XP_016448517.1HSF family protein
XP_016450204.1HSF family protein
XP_016453444.1HSF family protein
XP_016454588.1HSF family protein
XP_016455353.1HSF family protein
XP_016456461.1HSF family protein
XP_016465973.1HSF family protein
XP_016472860.1HSF family protein
XP_016472861.1HSF family protein
XP_016472862.1HSF family protein
XP_016472863.1HSF family protein
XP_016472864.1HSF family protein
XP_016473897.1HSF family protein
XP_016475994.1HSF family protein
XP_016478131.1HSF family protein
XP_016478371.1HSF family protein
XP_016478372.1HSF family protein
XP_016478373.1HSF family protein
XP_016478375.1HSF family protein
XP_016479025.1HSF family protein
XP_016479969.1HSF family protein
XP_016486569.1HSF family protein
XP_016486752.1HSF family protein
XP_016488254.1HSF family protein
XP_016488255.1HSF family protein
XP_016488450.1HSF family protein
XP_016489250.1HSF family protein
XP_016491218.1HSF family protein
XP_016493336.1HSF family protein
XP_016493667.1HSF family protein
XP_016496055.1HSF family protein
XP_016498663.1HSF family protein
XP_016498724.1HSF family protein
XP_016503096.1HSF family protein
XP_016503803.1HSF family protein
XP_016505243.1HSF family protein
XP_016505355.1HSF family protein
XP_016505356.1HSF family protein
XP_016506894.1HSF family protein
XP_016506895.1HSF family protein
XP_016512646.1HSF family protein
XP_016514409.1HSF family protein
XP_016514418.1HSF family protein
XP_016514635.1HSF family protein
XP_016515036.1HSF family protein
XP_016515037.1HSF family protein
XP_016515038.1HSF family protein
XP_016515943.1HSF family protein
XP_016515944.1HSF family protein
XP_016515945.1HSF family protein
XP_016515946.1HSF family protein
XP_016515947.1HSF family protein
XP_016515948.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645