PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Nicotiana sylvestris
HSF Family
Species TF ID Description
XP_009757456.1HSF family protein
XP_009757457.1HSF family protein
XP_009757458.1HSF family protein
XP_009757460.1HSF family protein
XP_009757461.1HSF family protein
XP_009757521.1HSF family protein
XP_009760921.1HSF family protein
XP_009765164.1HSF family protein
XP_009765951.1HSF family protein
XP_009765952.1HSF family protein
XP_009765953.1HSF family protein
XP_009765954.1HSF family protein
XP_009765955.1HSF family protein
XP_009769104.1HSF family protein
XP_009770587.1HSF family protein
XP_009770996.1HSF family protein
XP_009772330.1HSF family protein
XP_009775017.1HSF family protein
XP_009781208.1HSF family protein
XP_009783144.1HSF family protein
XP_009785112.1HSF family protein
XP_009785114.1HSF family protein
XP_009785115.1HSF family protein
XP_009785116.1HSF family protein
XP_009785117.1HSF family protein
XP_009785427.1HSF family protein
XP_009790212.1HSF family protein
XP_009790324.1HSF family protein
XP_009794990.1HSF family protein
XP_009796383.1HSF family protein
XP_009796384.1HSF family protein
XP_009796891.1HSF family protein
XP_009797496.1HSF family protein
XP_009797497.1HSF family protein
XP_009797839.1HSF family protein
XP_009798078.1HSF family protein
XP_009799323.1HSF family protein
XP_009799324.1HSF family protein
XP_009800135.1HSF family protein
XP_009802448.1HSF family protein
XP_009802449.1HSF family protein
XP_009803855.1HSF family protein
XP_009803856.1HSF family protein
XP_009803857.1HSF family protein
XP_009804280.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645