PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Nicotiana sylvestris
FAR1 Family
Species TF ID Description
XP_009758605.1FAR1 family protein
XP_009760738.1FAR1 family protein
XP_009761320.1FAR1 family protein
XP_009761563.1FAR1 family protein
XP_009761564.1FAR1 family protein
XP_009761565.1FAR1 family protein
XP_009761566.1FAR1 family protein
XP_009761567.1FAR1 family protein
XP_009761568.1FAR1 family protein
XP_009761570.1FAR1 family protein
XP_009761571.1FAR1 family protein
XP_009761907.1FAR1 family protein
XP_009762847.1FAR1 family protein
XP_009764129.1FAR1 family protein
XP_009764130.1FAR1 family protein
XP_009764131.1FAR1 family protein
XP_009764132.1FAR1 family protein
XP_009765793.1FAR1 family protein
XP_009765794.1FAR1 family protein
XP_009765927.1FAR1 family protein
XP_009765928.1FAR1 family protein
XP_009766776.1FAR1 family protein
XP_009767065.1FAR1 family protein
XP_009767066.1FAR1 family protein
XP_009767067.1FAR1 family protein
XP_009767068.1FAR1 family protein
XP_009768386.1FAR1 family protein
XP_009768387.1FAR1 family protein
XP_009768388.1FAR1 family protein
XP_009769143.1FAR1 family protein
XP_009771778.1FAR1 family protein
XP_009771804.1FAR1 family protein
XP_009772328.1FAR1 family protein
XP_009772337.1FAR1 family protein
XP_009772343.1FAR1 family protein
XP_009772350.1FAR1 family protein
XP_009772355.1FAR1 family protein
XP_009772359.1FAR1 family protein
XP_009772368.1FAR1 family protein
XP_009772373.1FAR1 family protein
XP_009772379.1FAR1 family protein
XP_009772385.1FAR1 family protein
XP_009772389.1FAR1 family protein
XP_009772395.1FAR1 family protein
XP_009772402.1FAR1 family protein
XP_009775261.1FAR1 family protein
XP_009776059.1FAR1 family protein
XP_009776060.1FAR1 family protein
XP_009776131.1FAR1 family protein
XP_009776132.1FAR1 family protein
XP_009776133.1FAR1 family protein
XP_009778050.1FAR1 family protein
XP_009779431.1FAR1 family protein
XP_009779432.1FAR1 family protein
XP_009779433.1FAR1 family protein
XP_009779434.1FAR1 family protein
XP_009779435.1FAR1 family protein
XP_009781959.1FAR1 family protein
XP_009783669.1FAR1 family protein
XP_009785478.1FAR1 family protein
XP_009785479.1FAR1 family protein
XP_009786841.1FAR1 family protein
XP_009786842.1FAR1 family protein
XP_009787995.1FAR1 family protein
XP_009788655.1FAR1 family protein
XP_009789344.1FAR1 family protein
XP_009791235.1FAR1 family protein
XP_009791236.1FAR1 family protein
XP_009791237.1FAR1 family protein
XP_009793957.1FAR1 family protein
XP_009797803.1FAR1 family protein
XP_009798613.1FAR1 family protein
XP_009798614.1FAR1 family protein
XP_009799718.1FAR1 family protein
XP_009801241.1FAR1 family protein
XP_009801242.1FAR1 family protein
XP_009801243.1FAR1 family protein
XP_009801244.1FAR1 family protein
XP_009802876.1FAR1 family protein
XP_009802877.1FAR1 family protein
XP_009804946.1FAR1 family protein
XP_009804947.1FAR1 family protein
XP_009804948.1FAR1 family protein
XP_009804949.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885