PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Malus domestica
HSF Family
Species TF ID Description
MDP0000119199HSF family protein
MDP0000126826HSF family protein
MDP0000127499HSF family protein
MDP0000129357HSF family protein
MDP0000148722HSF family protein
MDP0000150429HSF family protein
MDP0000155579HSF family protein
MDP0000155667HSF family protein
MDP0000155849HSF family protein
MDP0000156088HSF family protein
MDP0000156337HSF family protein
MDP0000163338HSF family protein
MDP0000172376HSF family protein
MDP0000174161HSF family protein
MDP0000185713HSF family protein
MDP0000190783HSF family protein
MDP0000191541HSF family protein
MDP0000194672HSF family protein
MDP0000202716HSF family protein
MDP0000209135HSF family protein
MDP0000216861HSF family protein
MDP0000230456HSF family protein
MDP0000232623HSF family protein
MDP0000239602HSF family protein
MDP0000243895HSF family protein
MDP0000253499HSF family protein
MDP0000254208HSF family protein
MDP0000260377HSF family protein
MDP0000263056HSF family protein
MDP0000273175HSF family protein
MDP0000291152HSF family protein
MDP0000301101HSF family protein
MDP0000302966HSF family protein
MDP0000319456HSF family protein
MDP0000319910HSF family protein
MDP0000320827HSF family protein
MDP0000325078HSF family protein
MDP0000456290HSF family protein
MDP0000485895HSF family protein
MDP0000489886HSF family protein
MDP0000498229HSF family protein
MDP0000527802HSF family protein
MDP0000589639HSF family protein
MDP0000606400HSF family protein
MDP0000613011HSF family protein
MDP0000622590HSF family protein
MDP0000746803HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645