PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Malus domestica
FAR1 Family
Species TF ID Description
MDP0000120171FAR1 family protein
MDP0000120361FAR1 family protein
MDP0000123745FAR1 family protein
MDP0000126939FAR1 family protein
MDP0000138209FAR1 family protein
MDP0000145320FAR1 family protein
MDP0000147190FAR1 family protein
MDP0000149037FAR1 family protein
MDP0000150696FAR1 family protein
MDP0000156730FAR1 family protein
MDP0000162123FAR1 family protein
MDP0000170251FAR1 family protein
MDP0000173549FAR1 family protein
MDP0000190269FAR1 family protein
MDP0000190656FAR1 family protein
MDP0000200105FAR1 family protein
MDP0000207355FAR1 family protein
MDP0000214642FAR1 family protein
MDP0000216756FAR1 family protein
MDP0000218395FAR1 family protein
MDP0000220752FAR1 family protein
MDP0000233216FAR1 family protein
MDP0000234814FAR1 family protein
MDP0000236134FAR1 family protein
MDP0000240375FAR1 family protein
MDP0000243502FAR1 family protein
MDP0000247737FAR1 family protein
MDP0000249284FAR1 family protein
MDP0000251291FAR1 family protein
MDP0000254218FAR1 family protein
MDP0000257646FAR1 family protein
MDP0000259867FAR1 family protein
MDP0000261185FAR1 family protein
MDP0000261311FAR1 family protein
MDP0000263830FAR1 family protein
MDP0000263959FAR1 family protein
MDP0000264383FAR1 family protein
MDP0000267783FAR1 family protein
MDP0000271754FAR1 family protein
MDP0000274034FAR1 family protein
MDP0000276468FAR1 family protein
MDP0000285475FAR1 family protein
MDP0000286803FAR1 family protein
MDP0000286804FAR1 family protein
MDP0000291883FAR1 family protein
MDP0000295749FAR1 family protein
MDP0000296271FAR1 family protein
MDP0000299726FAR1 family protein
MDP0000300795FAR1 family protein
MDP0000308670FAR1 family protein
MDP0000310682FAR1 family protein
MDP0000316852FAR1 family protein
MDP0000318097FAR1 family protein
MDP0000318339FAR1 family protein
MDP0000397753FAR1 family protein
MDP0000408462FAR1 family protein
MDP0000418901FAR1 family protein
MDP0000432072FAR1 family protein
MDP0000533379FAR1 family protein
MDP0000537221FAR1 family protein
MDP0000618200FAR1 family protein
MDP0000670522FAR1 family protein
MDP0000698017FAR1 family protein
MDP0000708770FAR1 family protein
MDP0000722905FAR1 family protein
MDP0000755159FAR1 family protein
MDP0000759009FAR1 family protein
MDP0000786741FAR1 family protein
MDP0000846736FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885