PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Leersia perrieri
HSF Family
Species TF ID Description
LPERR01G17270.1HSF family protein
LPERR01G20050.1HSF family protein
LPERR01G25670.1HSF family protein
LPERR01G26480.1HSF family protein
LPERR02G08320.1HSF family protein
LPERR02G13860.1HSF family protein
LPERR02G15050.1HSF family protein
LPERR03G04120.1HSF family protein
LPERR03G08060.1HSF family protein
LPERR03G08060.2HSF family protein
LPERR03G16010.1HSF family protein
LPERR03G28330.1HSF family protein
LPERR03G28330.2HSF family protein
LPERR03G31550.1HSF family protein
LPERR03G35390.1HSF family protein
LPERR04G18020.1HSF family protein
LPERR05G19500.1HSF family protein
LPERR06G14980.1HSF family protein
LPERR06G15480.1HSF family protein
LPERR07G04400.1HSF family protein
LPERR07G20810.1HSF family protein
LPERR08G14670.1HSF family protein
LPERR08G19290.1HSF family protein
LPERR09G09110.1HSF family protein
LPERR09G09190.1HSF family protein
LPERR09G13100.1HSF family protein
LPERR10G07380.1HSF family protein
LPERR10G07380.2HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645