PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Lotus japonicus
SBP Family
Species TF ID Description
Lj0g3v0057709.2SBP family protein
Lj0g3v0057709.3SBP family protein
Lj0g3v0140659.1SBP family protein
Lj0g3v0274819.1SBP family protein
Lj0g3v0274819.2SBP family protein
Lj0g3v0274819.3SBP family protein
Lj0g3v0301149.1SBP family protein
Lj0g3v0321769.1SBP family protein
Lj1g3v3847150.1SBP family protein
Lj1g3v3847150.2SBP family protein
Lj1g3v4830490.1SBP family protein
Lj2g3v1228810.1SBP family protein
Lj2g3v1228810.2SBP family protein
Lj2g3v3249980.1SBP family protein
Lj3g3v0429470.1SBP family protein
Lj3g3v0499510.1SBP family protein
Lj3g3v2809830.1SBP family protein
Lj3g3v2809830.2SBP family protein
Lj3g3v2809840.1SBP family protein
Lj4g3v0472820.1SBP family protein
Lj4g3v1725040.1SBP family protein
Lj4g3v2249130.1SBP family protein
Lj4g3v2249130.2SBP family protein
Lj4g3v2916450.1SBP family protein
Lj4g3v2916450.2SBP family protein
Lj4g3v2916450.3SBP family protein
Lj4g3v2916450.4SBP family protein
Lj5g3v1548910.1SBP family protein
Lj6g3v1916050.1SBP family protein
Lj6g3v2017480.1SBP family protein
SBP Family Introduction

SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Ishizuka Y, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors
J Mol Biol. 2004 Mar 12;337(1):49-63.
PMID: 15001351

SBP-box genes were first characterized as SQUAMOSA promoter binding proteins (SBPs) to regulate the expression of MADS-box genes in early flower development of A. majus (Klein et al., 1996). Since then, SBP-box genes have been identified in many plants including green alga, moss, silver birch, A. majus, Arabidopsis and maize. They play critical roles in regulating flower and fruit development as well as other physiological processes ([Moreno et al., 1997],[Eriksson et al., 2004], [Lannenpaa et al., 2004], [Arazi et al., 2005] and [Kropat et al., 2005]). It has been reported that Arabidopsis SPL3, SPL8 and SPL14 involves in flowering, sporogenesis, GA signaling and toxin resistance ([Cardon et al., 1997], [Unte et al., 2003], [Stone et al., 2005] and [Zhang et al., 2006]) while maize tga1 and tomato LeSPL-CNR affect fruit development ([Wang et al., 2005a] and [Manning et al., 2006]).

SBP-box genes encode proteins sharing a conserved DNA-binding domain of 79 amino acid residues. It has been proved that the DNA-binding domain of SBP-box genes is necessary and sufficient to bind to a palindromic GTAC core motif ([Klein et al., 1996], [Cardon et al., 1997], [Cardon et al., 1999], [Lannenpaa et al., 2004] and [Birkenbihl et al., 2005]). Studies on the NMR solution structure of the fragment of Arabidopsis SPL4 and SPL7 revealed that the DNA-binding domain of SBPs consisted of two separate zinc-binding sites. One zinc finger is C3H or C4 and the other is C2HC (Yamasaki et al., 2004 K. Yamasaki et al.,).

Guo AY, Zhu QH, Gu X, Ge S, Yang J, Luo J.
Genome-wide identification and evolutionary analysis of the plant specific SBP-box transcription factor family.
Gene. 2008 Jul 15;418(1-2):1-8.
PMID: 18495384