PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Kalanchoe laxiflora
HSF Family
Species TF ID Description
Kaladp0008s0762.1.pHSF family protein
Kaladp0008s0889.1.pHSF family protein
Kaladp0010s0021.1.pHSF family protein
Kaladp0010s0021.2.pHSF family protein
Kaladp0010s0067.1.pHSF family protein
Kaladp0011s0272.1.pHSF family protein
Kaladp0011s1224.1.pHSF family protein
Kaladp0012s0036.1.pHSF family protein
Kaladp0012s0036.2.pHSF family protein
Kaladp0012s0036.3.pHSF family protein
Kaladp0012s0036.4.pHSF family protein
Kaladp0012s0036.5.pHSF family protein
Kaladp0012s0036.6.pHSF family protein
Kaladp0015s0018.1.pHSF family protein
Kaladp0015s0018.2.pHSF family protein
Kaladp0015s0018.3.pHSF family protein
Kaladp0015s0018.4.pHSF family protein
Kaladp0024s0811.1.pHSF family protein
Kaladp0028s0067.1.pHSF family protein
Kaladp0031s0107.1.pHSF family protein
Kaladp0032s0071.1.pHSF family protein
Kaladp0034s0229.1.pHSF family protein
Kaladp0038s0140.1.pHSF family protein
Kaladp0039s0547.1.pHSF family protein
Kaladp0040s0240.1.pHSF family protein
Kaladp0040s0240.2.pHSF family protein
Kaladp0042s0072.1.pHSF family protein
Kaladp0048s0661.1.pHSF family protein
Kaladp0049s0010.1.pHSF family protein
Kaladp0053s0587.1.pHSF family protein
Kaladp0053s0587.2.pHSF family protein
Kaladp0053s0587.3.pHSF family protein
Kaladp0053s0587.4.pHSF family protein
Kaladp0055s0364.1.pHSF family protein
Kaladp0055s0364.2.pHSF family protein
Kaladp0055s0364.3.pHSF family protein
Kaladp0060s0167.1.pHSF family protein
Kaladp0064s0075.1.pHSF family protein
Kaladp0081s0271.1.pHSF family protein
Kaladp0082s0127.1.pHSF family protein
Kaladp0100s0025.1.pHSF family protein
Kaladp0101s0088.1.pHSF family protein
Kaladp0101s0088.2.pHSF family protein
Kaladp0102s0147.1.pHSF family protein
Kaladp0102s0147.2.pHSF family protein
Kaladp0102s0147.3.pHSF family protein
Kaladp0102s0147.4.pHSF family protein
Kaladp0102s0147.5.pHSF family protein
Kaladp0160s0001.1.pHSF family protein
Kaladp0427s0038.1.pHSF family protein
Kaladp0515s0230.1.pHSF family protein
Kaladp0808s0010.1.pHSF family protein
Kaladp0808s0010.2.pHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645