PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Juglans regia
FAR1 Family
Species TF ID Description
WALNUT_00002225-RAFAR1 family protein
WALNUT_00002348-RAFAR1 family protein
WALNUT_00002349-RAFAR1 family protein
WALNUT_00002350-RAFAR1 family protein
WALNUT_00002471-RAFAR1 family protein
WALNUT_00002472-RAFAR1 family protein
WALNUT_00003351-RAFAR1 family protein
WALNUT_00004376-RAFAR1 family protein
WALNUT_00004597-RAFAR1 family protein
WALNUT_00006036-RAFAR1 family protein
WALNUT_00006432-RAFAR1 family protein
WALNUT_00008378-RAFAR1 family protein
WALNUT_00009845-RAFAR1 family protein
WALNUT_00009970-RAFAR1 family protein
WALNUT_00011546-RAFAR1 family protein
WALNUT_00013301-RAFAR1 family protein
WALNUT_00013464-RAFAR1 family protein
WALNUT_00014502-RAFAR1 family protein
WALNUT_00016345-RAFAR1 family protein
WALNUT_00018283-RAFAR1 family protein
WALNUT_00018812-RAFAR1 family protein
WALNUT_00018813-RAFAR1 family protein
WALNUT_00018814-RAFAR1 family protein
WALNUT_00019357-RAFAR1 family protein
WALNUT_00020424-RAFAR1 family protein
WALNUT_00021256-RAFAR1 family protein
WALNUT_00022539-RAFAR1 family protein
WALNUT_00024331-RAFAR1 family protein
WALNUT_00028307-RAFAR1 family protein
WALNUT_00030882-RAFAR1 family protein
WALNUT_00031381-RAFAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885