PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Jatropha curcas
HSF Family
Species TF ID Description
Jcr4S00005.110HSF family protein
Jcr4S00021.40HSF family protein
Jcr4S00028.40HSF family protein
Jcr4S00150.190HSF family protein
Jcr4S00186.50HSF family protein
Jcr4S00197.90HSF family protein
Jcr4S00242.50HSF family protein
Jcr4S00335.100HSF family protein
Jcr4S00398.50HSF family protein
Jcr4S00929.30HSF family protein
Jcr4S01323.60HSF family protein
Jcr4S01324.10HSF family protein
Jcr4S03304.20HSF family protein
Jcr4S03964.20HSF family protein
Jcr4S04946.40HSF family protein
Jcr4S07357.10HSF family protein
Jcr4S11886.30HSF family protein
Jcr4S16816.10HSF family protein
Jcr4S22172.10HSF family protein
Jcr4S28273.10HSF family protein
Jcr4U32159.10HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645