PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Ipomoea trifida
HSF Family
Species TF ID Description
Itr_sc000013.1_g00024.1HSF family protein
Itr_sc000045.1_g00017.1HSF family protein
Itr_sc000055.1_g00006.1HSF family protein
Itr_sc000057.1_g00014.1HSF family protein
Itr_sc000060.1_g00007.1HSF family protein
Itr_sc000104.1_g00022.1HSF family protein
Itr_sc000104.1_g00023.1HSF family protein
Itr_sc000114.1_g00004.1HSF family protein
Itr_sc000122.1_g00014.1HSF family protein
Itr_sc000133.1_g00004.1HSF family protein
Itr_sc000220.1_g00001.1HSF family protein
Itr_sc000227.1_g00007.1HSF family protein
Itr_sc000269.1_g00015.1HSF family protein
Itr_sc000289.1_g00010.1HSF family protein
Itr_sc000327.1_g00036.1HSF family protein
Itr_sc000350.1_g00009.1HSF family protein
Itr_sc000415.1_g00013.1HSF family protein
Itr_sc000439.1_g00016.1HSF family protein
Itr_sc000526.1_g00011.1HSF family protein
Itr_sc000790.1_g00009.1HSF family protein
Itr_sc000879.1_g00012.1HSF family protein
Itr_sc000909.1_g00027.1HSF family protein
Itr_sc000918.1_g00017.1HSF family protein
Itr_sc000942.1_g00003.1HSF family protein
Itr_sc001111.1_g00017.1HSF family protein
Itr_sc001225.1_g00005.1HSF family protein
Itr_sc001598.1_g00007.1HSF family protein
Itr_sc001623.1_g00007.1HSF family protein
Itr_sc001623.1_g00008.1HSF family protein
Itr_sc002059.1_g00001.1HSF family protein
Itr_sc002113.1_g00005.1HSF family protein
Itr_sc005771.1_g00001.1HSF family protein
Itr_sc023973.1_g00001.1HSF family protein
Itr_sc060216.1_g00001.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645