PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Hordeum vulgare
HSF Family
Species TF ID Description
MLOC_11286.1HSF family protein
MLOC_11286.2HSF family protein
MLOC_11286.3HSF family protein
MLOC_11453.1HSF family protein
MLOC_11453.2HSF family protein
MLOC_21277.1HSF family protein
MLOC_498.3HSF family protein
MLOC_498.8HSF family protein
MLOC_498.9HSF family protein
MLOC_51191.1HSF family protein
MLOC_51191.2HSF family protein
MLOC_51191.3HSF family protein
MLOC_51191.4HSF family protein
MLOC_51191.6HSF family protein
MLOC_57709.1HSF family protein
MLOC_57709.3HSF family protein
MLOC_6206.3HSF family protein
MLOC_63532.1HSF family protein
MLOC_65182.1HSF family protein
MLOC_65182.2HSF family protein
MLOC_67158.1HSF family protein
MLOC_68417.1HSF family protein
MLOC_69675.2HSF family protein
MLOC_70567.1HSF family protein
MLOC_70567.2HSF family protein
MLOC_73300.1HSF family protein
MLOC_73300.2HSF family protein
MLOC_73356.1HSF family protein
MLOC_74270.1HSF family protein
MLOC_75166.4HSF family protein
MLOC_75519.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645