PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Glycine soja
HSF Family
Species TF ID Description
KHM99149.1HSF family protein
KHN01274.1HSF family protein
KHN02256.1HSF family protein
KHN02551.1HSF family protein
KHN02751.1HSF family protein
KHN03824.1HSF family protein
KHN04494.1HSF family protein
KHN04860.1HSF family protein
KHN06431.1HSF family protein
KHN06626.1HSF family protein
KHN07363.1HSF family protein
KHN10010.1HSF family protein
KHN10079.1HSF family protein
KHN10693.1HSF family protein
KHN12039.1HSF family protein
KHN12346.1HSF family protein
KHN12792.1HSF family protein
KHN14968.1HSF family protein
KHN15727.1HSF family protein
KHN16520.1HSF family protein
KHN17244.1HSF family protein
KHN17679.1HSF family protein
KHN18732.1HSF family protein
KHN21623.1HSF family protein
KHN21689.1HSF family protein
KHN22329.1HSF family protein
KHN27366.1HSF family protein
KHN28194.1HSF family protein
KHN28643.1HSF family protein
KHN29715.1HSF family protein
KHN31844.1HSF family protein
KHN34140.1HSF family protein
KHN35686.1HSF family protein
KHN36225.1HSF family protein
KHN37042.1HSF family protein
KHN38397.1HSF family protein
KHN39561.1HSF family protein
KHN40143.1HSF family protein
KHN42077.1HSF family protein
KHN45646.1HSF family protein
KHN47390.1HSF family protein
KHN48029.1HSF family protein
KHN48190.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645