PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Glycine soja
FAR1 Family
Species TF ID Description
KHN02803.1FAR1 family protein
KHN06125.1FAR1 family protein
KHN06202.1FAR1 family protein
KHN06792.1FAR1 family protein
KHN07370.1FAR1 family protein
KHN10095.1FAR1 family protein
KHN10135.1FAR1 family protein
KHN10555.1FAR1 family protein
KHN10918.1FAR1 family protein
KHN11326.1FAR1 family protein
KHN12906.1FAR1 family protein
KHN13933.1FAR1 family protein
KHN14374.1FAR1 family protein
KHN14375.1FAR1 family protein
KHN14976.1FAR1 family protein
KHN15674.1FAR1 family protein
KHN18250.1FAR1 family protein
KHN19983.1FAR1 family protein
KHN20508.1FAR1 family protein
KHN20602.1FAR1 family protein
KHN20822.1FAR1 family protein
KHN22170.1FAR1 family protein
KHN22392.1FAR1 family protein
KHN22805.1FAR1 family protein
KHN24804.1FAR1 family protein
KHN25102.1FAR1 family protein
KHN25297.1FAR1 family protein
KHN25302.1FAR1 family protein
KHN26435.1FAR1 family protein
KHN26808.1FAR1 family protein
KHN30100.1FAR1 family protein
KHN30121.1FAR1 family protein
KHN31358.1FAR1 family protein
KHN31427.1FAR1 family protein
KHN31867.1FAR1 family protein
KHN32681.1FAR1 family protein
KHN32934.1FAR1 family protein
KHN33085.1FAR1 family protein
KHN33306.1FAR1 family protein
KHN33310.1FAR1 family protein
KHN34514.1FAR1 family protein
KHN34778.1FAR1 family protein
KHN35527.1FAR1 family protein
KHN37548.1FAR1 family protein
KHN38124.1FAR1 family protein
KHN38445.1FAR1 family protein
KHN39540.1FAR1 family protein
KHN39704.1FAR1 family protein
KHN40737.1FAR1 family protein
KHN40804.1FAR1 family protein
KHN40913.1FAR1 family protein
KHN41795.1FAR1 family protein
KHN42330.1FAR1 family protein
KHN42873.1FAR1 family protein
KHN42936.1FAR1 family protein
KHN42959.1FAR1 family protein
KHN43827.1FAR1 family protein
KHN46338.1FAR1 family protein
KHN46858.1FAR1 family protein
KHN48773.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885