PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Eucalyptus camaldulensis
FAR1 Family
Species TF ID Description
EcC001316.20FAR1 family protein
EcC001753.10FAR1 family protein
EcC003157.10FAR1 family protein
EcC006223.20FAR1 family protein
EcC006223.40FAR1 family protein
EcC006223.60FAR1 family protein
EcC006588.20FAR1 family protein
EcC007322.40FAR1 family protein
EcC007367.30FAR1 family protein
EcC009842.30FAR1 family protein
EcC013663.30FAR1 family protein
EcC015072.40FAR1 family protein
EcC015787.10FAR1 family protein
EcC017141.20FAR1 family protein
EcC020645.20FAR1 family protein
EcC027143.20FAR1 family protein
EcC032205.20FAR1 family protein
EcC032446.10FAR1 family protein
EcC033088.20FAR1 family protein
EcC034086.30FAR1 family protein
EcC045276.30FAR1 family protein
EcC046871.50FAR1 family protein
EcC047086.40FAR1 family protein
EcC052648.30FAR1 family protein
EcC053935.30FAR1 family protein
EcC053990.70FAR1 family protein
EcC053990.90FAR1 family protein
EcC054762.100FAR1 family protein
EcC055193.10FAR1 family protein
EcC066213.10FAR1 family protein
EcC073440.10FAR1 family protein
EcC076057.10FAR1 family protein
EcC081225.10FAR1 family protein
EcS723945.10FAR1 family protein
EcS727201.10FAR1 family protein
EcS729352.10FAR1 family protein
EcS733210.10FAR1 family protein
EcS737708.10FAR1 family protein
EcS771492.10FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885