PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Citrus sinensis
FAR1 Family
Species TF ID Description
orange1.1g002062mFAR1 family protein
orange1.1g004138mFAR1 family protein
orange1.1g004420mFAR1 family protein
orange1.1g004429mFAR1 family protein
orange1.1g004450mFAR1 family protein
orange1.1g004769mFAR1 family protein
orange1.1g005549mFAR1 family protein
orange1.1g005560mFAR1 family protein
orange1.1g005579mFAR1 family protein
orange1.1g005927mFAR1 family protein
orange1.1g025532mFAR1 family protein
orange1.1g025623mFAR1 family protein
orange1.1g026704mFAR1 family protein
orange1.1g028041mFAR1 family protein
orange1.1g028717mFAR1 family protein
orange1.1g029989mFAR1 family protein
orange1.1g030419mFAR1 family protein
orange1.1g030575mFAR1 family protein
orange1.1g030584mFAR1 family protein
orange1.1g031272mFAR1 family protein
orange1.1g031637mFAR1 family protein
orange1.1g035792mFAR1 family protein
orange1.1g037026mFAR1 family protein
orange1.1g037828mFAR1 family protein
orange1.1g037987mFAR1 family protein
orange1.1g038176mFAR1 family protein
orange1.1g039289mFAR1 family protein
orange1.1g040872mFAR1 family protein
orange1.1g041847mFAR1 family protein
orange1.1g042400mFAR1 family protein
orange1.1g043090mFAR1 family protein
orange1.1g043648mFAR1 family protein
orange1.1g044894mFAR1 family protein
orange1.1g045204mFAR1 family protein
orange1.1g045678mFAR1 family protein
orange1.1g045718mFAR1 family protein
orange1.1g046293mFAR1 family protein
orange1.1g046611mFAR1 family protein
orange1.1g046720mFAR1 family protein
orange1.1g047253mFAR1 family protein
orange1.1g047580mFAR1 family protein
orange1.1g047657mFAR1 family protein
orange1.1g047780mFAR1 family protein
orange1.1g048670mFAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885