PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Cannabis sativa
FAR1 Family
Species TF ID Description
PK00134.1FAR1 family protein
PK00134.2FAR1 family protein
PK00134.3FAR1 family protein
PK00415.1FAR1 family protein
PK01244.1FAR1 family protein
PK01836.1FAR1 family protein
PK03582.1FAR1 family protein
PK04639.1FAR1 family protein
PK04639.2FAR1 family protein
PK05329.1FAR1 family protein
PK05401.1FAR1 family protein
PK05401.2FAR1 family protein
PK06148.1FAR1 family protein
PK06282.1FAR1 family protein
PK07165.1FAR1 family protein
PK07805.1FAR1 family protein
PK07805.2FAR1 family protein
PK07950.1FAR1 family protein
PK08039.1FAR1 family protein
PK08522.1FAR1 family protein
PK08903.1FAR1 family protein
PK09224.1FAR1 family protein
PK09224.2FAR1 family protein
PK10922.2FAR1 family protein
PK11838.1FAR1 family protein
PK12746.1FAR1 family protein
PK13380.1FAR1 family protein
PK15669.1FAR1 family protein
PK17289.1FAR1 family protein
PK19587.1FAR1 family protein
PK21037.1FAR1 family protein
PK22481.1FAR1 family protein
PK22885.1FAR1 family protein
PK25073.1FAR1 family protein
PK27567.1FAR1 family protein
PK27657.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885