PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Catharanthus roseus
FAR1 Family
Species TF ID Description
cra_locus_101999_iso_1FAR1 family protein
cra_locus_10287_iso_2FAR1 family protein
cra_locus_10287_iso_3FAR1 family protein
cra_locus_10287_iso_4FAR1 family protein
cra_locus_10417_iso_4FAR1 family protein
cra_locus_10782_iso_2FAR1 family protein
cra_locus_10782_iso_3FAR1 family protein
cra_locus_10782_iso_4FAR1 family protein
cra_locus_10782_iso_5FAR1 family protein
cra_locus_10782_iso_6FAR1 family protein
cra_locus_12781_iso_1FAR1 family protein
cra_locus_13295_iso_5FAR1 family protein
cra_locus_1415_iso_1FAR1 family protein
cra_locus_1415_iso_2FAR1 family protein
cra_locus_1415_iso_3FAR1 family protein
cra_locus_1415_iso_4FAR1 family protein
cra_locus_1415_iso_5FAR1 family protein
cra_locus_18283_iso_1FAR1 family protein
cra_locus_18283_iso_2FAR1 family protein
cra_locus_18283_iso_3FAR1 family protein
cra_locus_18283_iso_5FAR1 family protein
cra_locus_18283_iso_6FAR1 family protein
cra_locus_21529_iso_1FAR1 family protein
cra_locus_21529_iso_2FAR1 family protein
cra_locus_2213_iso_1FAR1 family protein
cra_locus_2213_iso_2FAR1 family protein
cra_locus_2333_iso_10FAR1 family protein
cra_locus_2333_iso_1FAR1 family protein
cra_locus_2333_iso_3FAR1 family protein
cra_locus_2333_iso_4FAR1 family protein
cra_locus_2333_iso_9FAR1 family protein
cra_locus_2595_iso_5FAR1 family protein
cra_locus_2881_iso_1FAR1 family protein
cra_locus_2881_iso_2FAR1 family protein
cra_locus_2881_iso_3FAR1 family protein
cra_locus_43041_iso_1FAR1 family protein
cra_locus_52887_iso_1FAR1 family protein
cra_locus_5316_iso_1FAR1 family protein
cra_locus_5316_iso_2FAR1 family protein
cra_locus_5316_iso_3FAR1 family protein
cra_locus_5316_iso_4FAR1 family protein
cra_locus_5316_iso_6FAR1 family protein
cra_locus_5316_iso_7FAR1 family protein
cra_locus_5487_iso_1FAR1 family protein
cra_locus_5487_iso_2FAR1 family protein
cra_locus_5487_iso_3FAR1 family protein
cra_locus_5487_iso_5FAR1 family protein
cra_locus_5487_iso_6FAR1 family protein
cra_locus_5487_iso_7FAR1 family protein
cra_locus_6732_iso_9FAR1 family protein
cra_locus_6851_iso_2FAR1 family protein
cra_locus_6851_iso_3FAR1 family protein
cra_locus_6851_iso_5FAR1 family protein
cra_locus_6851_iso_6FAR1 family protein
cra_locus_6928_iso_2FAR1 family protein
cra_locus_6928_iso_3FAR1 family protein
cra_locus_7149_iso_2FAR1 family protein
cra_locus_7149_iso_4FAR1 family protein
cra_locus_72631_iso_1FAR1 family protein
cra_locus_7331_iso_1FAR1 family protein
cra_locus_7532_iso_3FAR1 family protein
cra_locus_758_iso_2FAR1 family protein
cra_locus_7965_iso_1FAR1 family protein
cra_locus_7965_iso_3FAR1 family protein
cra_locus_8195_iso_2FAR1 family protein
cra_locus_8413_iso_1FAR1 family protein
cra_locus_8413_iso_2FAR1 family protein
cra_locus_8413_iso_3FAR1 family protein
cra_locus_8413_iso_4FAR1 family protein
cra_locus_8595_iso_1FAR1 family protein
cra_locus_8644_iso_9FAR1 family protein
cra_locus_9605_iso_1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885