PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Carica papaya
HSF Family
Species TF ID Description
evm.model.supercontig_107.31HSF family protein
evm.model.supercontig_124.4HSF family protein
evm.model.supercontig_157.44HSF family protein
evm.model.supercontig_1780.1HSF family protein
evm.model.supercontig_2.169HSF family protein
evm.model.supercontig_224.10HSF family protein
evm.model.supercontig_27.46HSF family protein
evm.model.supercontig_28.162HSF family protein
evm.model.supercontig_43.94HSF family protein
evm.model.supercontig_44.122HSF family protein
evm.model.supercontig_49.32HSF family protein
evm.model.supercontig_50.58HSF family protein
evm.model.supercontig_52.116HSF family protein
evm.model.supercontig_55.150HSF family protein
evm.model.supercontig_5.77HSF family protein
evm.model.supercontig_7.163HSF family protein
evm.model.supercontig_74.64HSF family protein
evm.model.supercontig_90.16HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645