PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Citrullus lanatus
ZF-HD Family
Species TF ID Description
Cla001881ZF-HD family protein
Cla004862ZF-HD family protein
Cla010184ZF-HD family protein
Cla010330ZF-HD family protein
Cla010331ZF-HD family protein
Cla014118ZF-HD family protein
Cla014215ZF-HD family protein
Cla014583ZF-HD family protein
Cla017011ZF-HD family protein
Cla018430ZF-HD family protein
Cla018609ZF-HD family protein
Cla019114ZF-HD family protein
Cla022132ZF-HD family protein
Cla022489ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511