PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Cajanus cajan
HSF Family
Species TF ID Description
C.cajan_00661HSF family protein
C.cajan_01041HSF family protein
C.cajan_03188HSF family protein
C.cajan_03745HSF family protein
C.cajan_05711HSF family protein
C.cajan_06276HSF family protein
C.cajan_07385HSF family protein
C.cajan_07476HSF family protein
C.cajan_09277HSF family protein
C.cajan_09456HSF family protein
C.cajan_12769HSF family protein
C.cajan_13182HSF family protein
C.cajan_15551HSF family protein
C.cajan_19915HSF family protein
C.cajan_24088HSF family protein
C.cajan_25281HSF family protein
C.cajan_27606HSF family protein
C.cajan_28926HSF family protein
C.cajan_29530HSF family protein
C.cajan_30373HSF family protein
C.cajan_30804HSF family protein
C.cajan_32266HSF family protein
C.cajan_33410HSF family protein
C.cajan_36071HSF family protein
C.cajan_36375HSF family protein
C.cajan_37173HSF family protein
C.cajan_44684HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645