PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Camelina sativa
FAR1 Family
Species TF ID Description
Csa00430s030.1FAR1 family protein
Csa01g007260.1FAR1 family protein
Csa01g008520.1FAR1 family protein
Csa01g025390.1FAR1 family protein
Csa02317s010.1FAR1 family protein
Csa03g013650.1FAR1 family protein
Csa03g051450.1FAR1 family protein
Csa03g059500.1FAR1 family protein
Csa04g015670.1FAR1 family protein
Csa04g060890.1FAR1 family protein
Csa05g007190.1FAR1 family protein
Csa05g025760.1FAR1 family protein
Csa05g041940.1FAR1 family protein
Csa05g090000.1FAR1 family protein
Csa06g049510.1FAR1 family protein
Csa07g006690.1FAR1 family protein
Csa07g022030.1FAR1 family protein
Csa07g047860.1FAR1 family protein
Csa07g059860.1FAR1 family protein
Csa07g060670.1FAR1 family protein
Csa08g011480.1FAR1 family protein
Csa08g029960.1FAR1 family protein
Csa09g081030.1FAR1 family protein
Csa09g095030.1FAR1 family protein
Csa10g023200.1FAR1 family protein
Csa10g031150.1FAR1 family protein
Csa11g001070.1FAR1 family protein
Csa11g001080.1FAR1 family protein
Csa11g026250.1FAR1 family protein
Csa11g033300.1FAR1 family protein
Csa11g035510.1FAR1 family protein
Csa11g089420.1FAR1 family protein
Csa12g001000.1FAR1 family protein
Csa12g039390.1FAR1 family protein
Csa12g039390.2FAR1 family protein
Csa13g017150.1FAR1 family protein
Csa13g021800.1FAR1 family protein
Csa13g038050.1FAR1 family protein
Csa13g041910.1FAR1 family protein
Csa14g011720.1FAR1 family protein
Csa14g061770.1FAR1 family protein
Csa15g007820.1FAR1 family protein
Csa15g009230.1FAR1 family protein
Csa15g034750.1FAR1 family protein
Csa16g006480.1FAR1 family protein
Csa16g040450.1FAR1 family protein
Csa16g051270.1FAR1 family protein
Csa17g013860.1FAR1 family protein
Csa17g092510.1FAR1 family protein
Csa19g009920.1FAR1 family protein
Csa19g011260.1FAR1 family protein
Csa19g031380.1FAR1 family protein
Csa20g027400.1FAR1 family protein
Csa20g057530.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885