PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Capsicum annuum
HSF Family
Species TF ID Description
CA00g45390HSF family protein
CA00g63000HSF family protein
CA00g71530HSF family protein
CA01g03100HSF family protein
CA01g07540HSF family protein
CA01g30350HSF family protein
CA02g11030HSF family protein
CA02g13280HSF family protein
CA02g13320HSF family protein
CA02g16000HSF family protein
CA02g16840HSF family protein
CA03g06850HSF family protein
CA03g11650HSF family protein
CA03g16300HSF family protein
CA03g21660HSF family protein
CA04g01070HSF family protein
CA04g18550HSF family protein
CA05g00840HSF family protein
CA06g08710HSF family protein
CA07g15920HSF family protein
CA08g05000HSF family protein
CA09g01450HSF family protein
CA09g11190HSF family protein
CA10g20440HSF family protein
CA12g20590HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645