PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Brachypodium stacei
FAR1 Family
Species TF ID Description
Brast01G162100.1.pFAR1 family protein
Brast01G252100.1.pFAR1 family protein
Brast01G342900.1.pFAR1 family protein
Brast02G119100.1.pFAR1 family protein
Brast02G119400.1.pFAR1 family protein
Brast02G215100.1.pFAR1 family protein
Brast02G248400.1.pFAR1 family protein
Brast02G252100.1.pFAR1 family protein
Brast02G264700.1.pFAR1 family protein
Brast02G325900.1.pFAR1 family protein
Brast02G325900.2.pFAR1 family protein
Brast02G379700.1.pFAR1 family protein
Brast02G379700.2.pFAR1 family protein
Brast03G022400.1.pFAR1 family protein
Brast03G117800.1.pFAR1 family protein
Brast03G119400.1.pFAR1 family protein
Brast03G119500.1.pFAR1 family protein
Brast04G038400.1.pFAR1 family protein
Brast04G040400.1.pFAR1 family protein
Brast04G076500.1.pFAR1 family protein
Brast04G107300.1.pFAR1 family protein
Brast04G142900.1.pFAR1 family protein
Brast04G143200.1.pFAR1 family protein
Brast04G175100.1.pFAR1 family protein
Brast04G198100.1.pFAR1 family protein
Brast04G217700.1.pFAR1 family protein
Brast04G217800.1.pFAR1 family protein
Brast04G217800.2.pFAR1 family protein
Brast04G217800.3.pFAR1 family protein
Brast04G217800.4.pFAR1 family protein
Brast04G218000.1.pFAR1 family protein
Brast04G218000.2.pFAR1 family protein
Brast04G218100.1.pFAR1 family protein
Brast04G218100.2.pFAR1 family protein
Brast04G324800.1.pFAR1 family protein
Brast05G109700.1.pFAR1 family protein
Brast05G129800.1.pFAR1 family protein
Brast05G136600.1.pFAR1 family protein
Brast05G170700.1.pFAR1 family protein
Brast05G239700.1.pFAR1 family protein
Brast05G262500.1.pFAR1 family protein
Brast05G277500.1.pFAR1 family protein
Brast06G047200.1.pFAR1 family protein
Brast06G047200.2.pFAR1 family protein
Brast06G092000.1.pFAR1 family protein
Brast06G120700.1.pFAR1 family protein
Brast06G120900.1.pFAR1 family protein
Brast06G120900.2.pFAR1 family protein
Brast06G165200.1.pFAR1 family protein
Brast06G175500.1.pFAR1 family protein
Brast07G045700.1.pFAR1 family protein
Brast07G096100.1.pFAR1 family protein
Brast07G140100.1.pFAR1 family protein
Brast07G148600.1.pFAR1 family protein
Brast07G159300.1.pFAR1 family protein
Brast07G159300.2.pFAR1 family protein
Brast07G246200.1.pFAR1 family protein
Brast08G085900.1.pFAR1 family protein
Brast08G101400.1.pFAR1 family protein
Brast08G170700.1.pFAR1 family protein
Brast08G183200.1.pFAR1 family protein
Brast09G036400.1.pFAR1 family protein
Brast09G044000.1.pFAR1 family protein
Brast09G092500.1.pFAR1 family protein
Brast09G092500.2.pFAR1 family protein
Brast09G092500.3.pFAR1 family protein
Brast09G118400.1.pFAR1 family protein
Brast09G118400.2.pFAR1 family protein
Brast09G208700.1.pFAR1 family protein
Brast09G208700.2.pFAR1 family protein
Brast10G087800.1.pFAR1 family protein
Brast10G097900.1.pFAR1 family protein
Brast10G097900.2.pFAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885