PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Brassica rapa
ZF-HD Family
Species TF ID Description
XP_009104712.1ZF-HD family protein
XP_009104753.1ZF-HD family protein
XP_009106129.1ZF-HD family protein
XP_009106180.1ZF-HD family protein
XP_009109241.1ZF-HD family protein
XP_009110317.1ZF-HD family protein
XP_009111614.1ZF-HD family protein
XP_009111615.1ZF-HD family protein
XP_009112180.1ZF-HD family protein
XP_009112487.1ZF-HD family protein
XP_009114039.1ZF-HD family protein
XP_009115766.1ZF-HD family protein
XP_009115767.1ZF-HD family protein
XP_009117583.1ZF-HD family protein
XP_009117884.1ZF-HD family protein
XP_009117900.1ZF-HD family protein
XP_009117901.1ZF-HD family protein
XP_009121598.1ZF-HD family protein
XP_009127747.1ZF-HD family protein
XP_009128086.1ZF-HD family protein
XP_009129138.1ZF-HD family protein
XP_009130511.1ZF-HD family protein
XP_009131913.1ZF-HD family protein
XP_009136567.1ZF-HD family protein
XP_009136568.1ZF-HD family protein
XP_009136569.1ZF-HD family protein
XP_009138327.1ZF-HD family protein
XP_009139922.1ZF-HD family protein
XP_009148819.1ZF-HD family protein
XP_009149365.1ZF-HD family protein
XP_009150629.1ZF-HD family protein
XP_009151829.1ZF-HD family protein
XP_009151830.1ZF-HD family protein
XP_009151831.1ZF-HD family protein
XP_009151833.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511