PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Brassica rapa
HSF Family
Species TF ID Description
XP_009102028.1HSF family protein
XP_009102502.1HSF family protein
XP_009105347.1HSF family protein
XP_009107442.1HSF family protein
XP_009107990.1HSF family protein
XP_009108394.1HSF family protein
XP_009108395.1HSF family protein
XP_009108396.1HSF family protein
XP_009108632.1HSF family protein
XP_009114395.1HSF family protein
XP_009114396.1HSF family protein
XP_009114894.1HSF family protein
XP_009116993.1HSF family protein
XP_009118959.1HSF family protein
XP_009119797.1HSF family protein
XP_009120089.1HSF family protein
XP_009121441.1HSF family protein
XP_009121442.1HSF family protein
XP_009122679.1HSF family protein
XP_009123909.1HSF family protein
XP_009124795.1HSF family protein
XP_009126142.1HSF family protein
XP_009130189.1HSF family protein
XP_009130190.1HSF family protein
XP_009130314.1HSF family protein
XP_009130326.1HSF family protein
XP_009130935.1HSF family protein
XP_009132283.1HSF family protein
XP_009132288.1HSF family protein
XP_009132950.1HSF family protein
XP_009132988.1HSF family protein
XP_009133062.1HSF family protein
XP_009133068.1HSF family protein
XP_009133079.1HSF family protein
XP_009133506.1HSF family protein
XP_009133967.1HSF family protein
XP_009134244.1HSF family protein
XP_009134718.1HSF family protein
XP_009135829.1HSF family protein
XP_009136076.1HSF family protein
XP_009136642.1HSF family protein
XP_009136643.1HSF family protein
XP_009136968.1HSF family protein
XP_009137089.1HSF family protein
XP_009137759.1HSF family protein
XP_009138469.1HSF family protein
XP_009138768.1HSF family protein
XP_009139495.1HSF family protein
XP_009139571.1HSF family protein
XP_009139572.1HSF family protein
XP_009140178.1HSF family protein
XP_009140184.1HSF family protein
XP_009140185.1HSF family protein
XP_009140186.1HSF family protein
XP_009140412.1HSF family protein
XP_009141897.1HSF family protein
XP_009141898.1HSF family protein
XP_009141900.1HSF family protein
XP_009141901.1HSF family protein
XP_009141902.1HSF family protein
XP_009145259.1HSF family protein
XP_009151322.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645