PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Brassica oleracea
HSF Family
Species TF ID Description
XP_013583761.1HSF family protein
XP_013584623.1HSF family protein
XP_013586097.1HSF family protein
XP_013588127.1HSF family protein
XP_013588180.1HSF family protein
XP_013589911.1HSF family protein
XP_013593227.1HSF family protein
XP_013593957.1HSF family protein
XP_013595493.1HSF family protein
XP_013596160.1HSF family protein
XP_013596620.1HSF family protein
XP_013599385.1HSF family protein
XP_013603200.1HSF family protein
XP_013603496.1HSF family protein
XP_013603497.1HSF family protein
XP_013606095.1HSF family protein
XP_013606096.1HSF family protein
XP_013606495.1HSF family protein
XP_013606496.1HSF family protein
XP_013606497.1HSF family protein
XP_013611350.1HSF family protein
XP_013611351.1HSF family protein
XP_013611352.1HSF family protein
XP_013611353.1HSF family protein
XP_013611354.1HSF family protein
XP_013611767.1HSF family protein
XP_013613166.1HSF family protein
XP_013613167.1HSF family protein
XP_013614420.1HSF family protein
XP_013614421.1HSF family protein
XP_013615227.1HSF family protein
XP_013615677.1HSF family protein
XP_013617634.1HSF family protein
XP_013617733.1HSF family protein
XP_013618084.1HSF family protein
XP_013618730.1HSF family protein
XP_013621550.1HSF family protein
XP_013621685.1HSF family protein
XP_013621686.1HSF family protein
XP_013622445.1HSF family protein
XP_013622640.1HSF family protein
XP_013623223.1HSF family protein
XP_013623224.1HSF family protein
XP_013623225.1HSF family protein
XP_013623226.1HSF family protein
XP_013624421.1HSF family protein
XP_013625932.1HSF family protein
XP_013628204.1HSF family protein
XP_013629747.1HSF family protein
XP_013630574.1HSF family protein
XP_013630575.1HSF family protein
XP_013631217.1HSF family protein
XP_013633144.1HSF family protein
XP_013633148.1HSF family protein
XP_013633154.1HSF family protein
XP_013633434.1HSF family protein
XP_013636305.1HSF family protein
XP_013636306.1HSF family protein
XP_013636307.1HSF family protein
XP_013636308.1HSF family protein
XP_013636309.1HSF family protein
XP_013636310.1HSF family protein
XP_013637590.1HSF family protein
XP_013637593.1HSF family protein
XP_013638778.1HSF family protein
XP_013639669.1HSF family protein
XP_013639709.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645