PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Brassica napus
HSF Family
Species TF ID Description
GSBRNA2T00001515001HSF family protein
GSBRNA2T00011397001HSF family protein
GSBRNA2T00012182001HSF family protein
GSBRNA2T00015936001HSF family protein
GSBRNA2T00019126001HSF family protein
GSBRNA2T00021295001HSF family protein
GSBRNA2T00021594001HSF family protein
GSBRNA2T00024833001HSF family protein
GSBRNA2T00026029001HSF family protein
GSBRNA2T00030047001HSF family protein
GSBRNA2T00030079001HSF family protein
GSBRNA2T00032165001HSF family protein
GSBRNA2T00032261001HSF family protein
GSBRNA2T00035335001HSF family protein
GSBRNA2T00040967001HSF family protein
GSBRNA2T00041369001HSF family protein
GSBRNA2T00041793001HSF family protein
GSBRNA2T00044326001HSF family protein
GSBRNA2T00045453001HSF family protein
GSBRNA2T00045458001HSF family protein
GSBRNA2T00046846001HSF family protein
GSBRNA2T00048439001HSF family protein
GSBRNA2T00049541001HSF family protein
GSBRNA2T00052589001HSF family protein
GSBRNA2T00056228001HSF family protein
GSBRNA2T00058391001HSF family protein
GSBRNA2T00060505001HSF family protein
GSBRNA2T00062653001HSF family protein
GSBRNA2T00062666001HSF family protein
GSBRNA2T00065352001HSF family protein
GSBRNA2T00066805001HSF family protein
GSBRNA2T00068086001HSF family protein
GSBRNA2T00070282001HSF family protein
GSBRNA2T00070860001HSF family protein
GSBRNA2T00071800001HSF family protein
GSBRNA2T00075260001HSF family protein
GSBRNA2T00076133001HSF family protein
GSBRNA2T00080527001HSF family protein
GSBRNA2T00080529001HSF family protein
GSBRNA2T00080534001HSF family protein
GSBRNA2T00080614001HSF family protein
GSBRNA2T00084799001HSF family protein
GSBRNA2T00085186001HSF family protein
GSBRNA2T00085879001HSF family protein
GSBRNA2T00085882001HSF family protein
GSBRNA2T00090310001HSF family protein
GSBRNA2T00091010001HSF family protein
GSBRNA2T00092794001HSF family protein
GSBRNA2T00092962001HSF family protein
GSBRNA2T00094912001HSF family protein
GSBRNA2T00095023001HSF family protein
GSBRNA2T00095130001HSF family protein
GSBRNA2T00095456001HSF family protein
GSBRNA2T00097134001HSF family protein
GSBRNA2T00100979001HSF family protein
GSBRNA2T00102095001HSF family protein
GSBRNA2T00107662001HSF family protein
GSBRNA2T00107744001HSF family protein
GSBRNA2T00108159001HSF family protein
GSBRNA2T00109796001HSF family protein
GSBRNA2T00111272001HSF family protein
GSBRNA2T00111477001HSF family protein
GSBRNA2T00112009001HSF family protein
GSBRNA2T00112601001HSF family protein
GSBRNA2T00113059001HSF family protein
GSBRNA2T00114270001HSF family protein
GSBRNA2T00122982001HSF family protein
GSBRNA2T00123298001HSF family protein
GSBRNA2T00123300001HSF family protein
GSBRNA2T00126929001HSF family protein
GSBRNA2T00127177001HSF family protein
GSBRNA2T00128264001HSF family protein
GSBRNA2T00130822001HSF family protein
GSBRNA2T00131093001HSF family protein
GSBRNA2T00131260001HSF family protein
GSBRNA2T00131388001HSF family protein
GSBRNA2T00131442001HSF family protein
GSBRNA2T00131447001HSF family protein
GSBRNA2T00135387001HSF family protein
GSBRNA2T00136440001HSF family protein
GSBRNA2T00138764001HSF family protein
GSBRNA2T00141953001HSF family protein
GSBRNA2T00143186001HSF family protein
GSBRNA2T00145482001HSF family protein
GSBRNA2T00145751001HSF family protein
GSBRNA2T00151270001HSF family protein
GSBRNA2T00151271001HSF family protein
GSBRNA2T00151511001HSF family protein
GSBRNA2T00153686001HSF family protein
GSBRNA2T00154690001HSF family protein
GSBRNA2T00154829001HSF family protein
GSBRNA2T00154915001HSF family protein
GSBRNA2T00156634001HSF family protein
GSBRNA2T00158438001HSF family protein
GSBRNA2T00158442001HSF family protein
GSBRNA2T00158445001HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645