PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Brachypodium distachyon
HSF Family
Species TF ID Description
Bradi1g01130.1.pHSF family protein
Bradi1g01130.2.pHSF family protein
Bradi1g05550.1.pHSF family protein
Bradi1g08891.1.pHSF family protein
Bradi1g08891.2.pHSF family protein
Bradi1g08891.3.pHSF family protein
Bradi1g08891.4.pHSF family protein
Bradi1g08891.5.pHSF family protein
Bradi1g08891.6.pHSF family protein
Bradi1g19900.1.pHSF family protein
Bradi1g19900.2.pHSF family protein
Bradi1g37720.1.pHSF family protein
Bradi1g38140.1.pHSF family protein
Bradi1g38140.2.pHSF family protein
Bradi1g55630.2.pHSF family protein
Bradi1g55630.3.pHSF family protein
Bradi1g61620.1.pHSF family protein
Bradi1g61620.2.pHSF family protein
Bradi1g69407.1.pHSF family protein
Bradi1g74350.2.pHSF family protein
Bradi1g74350.3.pHSF family protein
Bradi2g18980.2.pHSF family protein
Bradi2g18980.3.pHSF family protein
Bradi2g18980.4.pHSF family protein
Bradi2g41530.1.pHSF family protein
Bradi2g44050.1.pHSF family protein
Bradi2g44050.2.pHSF family protein
Bradi2g48990.1.pHSF family protein
Bradi2g49860.1.pHSF family protein
Bradi2g49860.3.pHSF family protein
Bradi2g49860.4.pHSF family protein
Bradi3g08870.1.pHSF family protein
Bradi3g26920.4.pHSF family protein
Bradi3g26920.6.pHSF family protein
Bradi3g26920.8.pHSF family protein
Bradi3g42130.1.pHSF family protein
Bradi3g43710.1.pHSF family protein
Bradi3g44700.1.pHSF family protein
Bradi4g32050.1.pHSF family protein
Bradi4g32130.1.pHSF family protein
Bradi4g35780.3.pHSF family protein
Bradi5g18680.1.pHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645